You are viewing an incomplete version of our website. Please click to reload the website as full version.

CD56 antibody (Neural Cell Adhesion Molecule 1) (APC)

Details for Product anti-NCAM1 Antibody No. ABIN94191, Supplier: Log in to see
Antigen
  • Cd56
  • CD56
  • E-NCAM
  • fj56g09
  • MSK39
  • N-CAM
  • n-cam
  • N-CAM-1
  • Ncam
  • ncam
  • NCAM
  • NCAM-1
  • NCAM-C
  • ncam1
  • NCAM1
  • NCAMC
  • wu:fj56g09
Reactivity
Human, Non-Human Primate
601
93
72
21
8
8
7
4
4
3
3
3
3
2
1
Host
Mouse
523
97
14
11
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD56 antibody is conjugated to APC
60
53
39
27
9
9
8
8
7
6
6
6
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
348
238
122
117
96
81
75
55
23
22
7
6
5
4
4
3
3
2
2
2
2
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen KG-1 human acute myelogenous leukemia cell line
Clone MEM-188
Isotype IgG2a
Specificity The antibody MEM-188 reacts with a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested. HLDA VI, WS code A055HLDA VI, WS Code NK26HLDA VII, WS code 70077
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Alternative Name NCAM1 (NCAM1 Antibody Abstract)
Background CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Supplier Images
Flow Cytometry (FACS) image for anti-CD56 antibody (Neural Cell Adhesion Molecule 1)  (APC) (ABIN94191) Surface staining of human peripheral blood cells with anti-CD56 (MEM-188) FITC.
Product cited in: Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, 2011

Benson, Grobarova, Richter, Fiserova: "Glycosylation regulates NK cell-mediated effector function through PI3K pathway." in: International immunology, Vol. 22, Issue 3, pp. 167-77, 2010

Olsen, Tollefsen, Aagaard, Reitan, Bannantine, Andersen, Sollid, Lundin: "Isolation of Mycobacterium avium subspecies paratuberculosis reactive CD4 T cells from intestinal biopsies of Crohn's disease patients." in: PLoS ONE, Vol. 4, Issue 5, pp. e5641, 2009

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007

Yates, Rovis, Mitchell, Afzali, Tsang, Garin, Lechler, Lombardi, Garden: "The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro." in: International immunology, Vol. 19, Issue 6, pp. 785-99, 2007

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002

Background publications McCluggage, McKenna, McBride: "CD56 is a sensitive and diagnostically useful immunohistochemical marker of ovarian sex cord-stromal tumors." in: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, Vol. 26, Issue 3, pp. 322-7, 2007

Ohishi, Kaku, Oya, Kobayashi, Wake, Tsuneyoshi: "CD56 expression in ovarian granulosa cell tumors, and its diagnostic utility and pitfalls." in: Gynecologic oncology, Vol. 107, Issue 1, pp. 30-8, 2007

Jakovcevski, Mo, Zecevic: "Down-regulation of the axonal polysialic acid-neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain." in: Neuroscience, Vol. 149, Issue 2, pp. 328-37, 2007

Lin, Liu, Chen, Wang, Medeiros, Hsu: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005

Lanier, Chang, Azuma, Ruitenberg, Hemperly, Phillips: "Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 12, pp. 4421-6, 1991