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Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibody
| Antigen | Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) |
| Synonyms | P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1, CDKI, Waf1, mda6, Cdkn1, p21WAF, p21Cip1, Cip1, CDKN1A, p21, cip1, p16Xic2, sdi1, waf1, cap20, cdkn1, mda-6, p21cip1, si:ch1073-48m5.2 |
| Clonality | Monoclonal (WA-1) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human, Primates |
| Application |
Alternatives Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunocytochemistry (ICC)
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| Catalog no. | ABIN94444 |
| Quantity | 0.1 mg (1 mg/ml) (Variants) |
| Price | 286.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 3 to 5 Business Days |
Additional Information
| Alternative name | p21Waf1 |
| Immunogen | Human p21 protein |
| Format | Purified |
| Isotype | IgG1 (Matching secondary antibodies) |
| Clone | WA-1 |
| Description | The tumour suppressor p21Waf1 (Wild-type p53-Activated Fragment 1, also known as Cip1,Cdk interacting protein, or SDI 1) is a cyclin-dependent kinase (Cdk) inhibitor, which isexpressed by involvement of p53, Egr-1, AP2, STATs or other transcription factors uponvarious stimuli resulting in cell cycle arrest. Through its N-terminal domain p21Waf1 inhibitsCdk activity, whereas through the C-terminal domain it inhibits the activity of PCNA(proliferating cell nuclear antigen) to activate DNA replication. Cytosolic location of p21counteracts its inhibitory activities. |
| Specificity | The antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1), a 21 kDa tumoursuppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferatingcell nuclear antigen. |
Application Details
| Application Notes | Immunohistochemistry (paraffin sections) - Positive tissue: colon carcinomaImmunocytochemistryELISA |
| Concentration | 1 mg/ml |
| Purity | > 95% (by SDS-PAGE) |
| Purification | Purified from ascites by precipitation methods. |
| Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage | Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles. |
| Restrictions | For Research Use only |
Images
| Immunohistochemistry staining of colon carcinoma (paraffin-embedded sections) with anti-p21 (WA-1). |
Alternatives
Alternatives for antigen "Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A)", type "Antibodies"
| Hosts | Rabbit (43), Mouse (23) |
| Reactivities | Human (60), Rat (Rattus) (8), Mouse (Murine) (6), Mammalian (2), Cow (Bovine) (1), Sheep (Ovine) (1) |
| Applications | Western Blotting (WB) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Enzyme Immunoassay (EIA) (18), Immunohistochemistry (IHC) (14), ELISA (10), Immunoprecipitation (IP) (6), Dot Blot (Dot) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunofluorescence (IF) (3), Flow Cytometry (FACS) (2), ELISA (Detection) (1) |
| Epitopes | pThr145 (10), Ab 145 (4), C-Term (4), pSer130 (2), pThr57 (2), pS130 (1), pSer146 (1), pSer58 (1), pT145 (1), pT57 (1) |
