DFFB antibody (N-Term)

Catalog No. ABIN951896

Product Details anti-DFFB Antibody

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Binding Specificity: AA 1-30, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DFFB antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody recognizes Human DFFB / CAD (N-term).

Purification: Affinity Chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of Human DFFB / CAD. Genename: DFFB

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DFFB

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Alternative Name: DFFB / CAD (DFFB Products)

Synonyms: CAD antibody, CPAN antibody, DFF-40 antibody, DFF2 antibody, DFF40 antibody, LOC100223514 antibody, 40kDa antibody, 5730477D02Rik antibody, Didff antibody, Cad antibody, carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase antibody, DNA fragmentation factor subunit beta antibody, DNA fragmentation factor, beta polypeptide (caspase-activated DNase) antibody, DNA fragmentation factor, beta subunit antibody, CAD antibody, DFFB antibody, dffb antibody, LOC100223514 antibody, Dffb antibody

Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosisSynonyms: CPAN, Caspase-activated DNase, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DFF-40, DFF2, DFF40, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor subunit beta

Molecular Weight: 39110 Da

Gene ID: 1677

NCBI Accession: NP_004393

Pathways: Apoptosis, Caspase Cascade in Apoptosis