MAP3K4 antibody (Middle Region)

Catalog No. ABIN953298

This anti-MAP3K4 antibody is a Rabbit Polyclonal antibody detecting MAP3K4 in WB, IHC (p) and EIA. Suitable for Human.

Quick Overview for MAP3K4 antibody (Middle Region) (ABIN953298)

Target: MAP3K4 (Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-MAP3K4 Antibody

Binding Specificity: AA 1065-1097, Middle Region

Specificity: This antibody reacts to MEKK4.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 1065-1097 amino acids from the Central region of human MEKK4 Genename: MAP3K4

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MAP3K4

Target: MAP3K4 (Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4))

Alternative Name: MAP3K4

Background: The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described.Synonyms: MAP three kinase 1, MAPK/ERK kinase kinase 4, MAPKKK4, MEKK4, MTK1, Mitogen-activated protein kinase kinase kinase 4

Molecular Weight: 181685 Da

Gene ID: 4216

NCBI Accession: NP_005913

Pathways: MAPK Signaling