You are viewing an incomplete version of our website. Please click to reload the website as full version.

Mediator Complex Subunit 6 (MED6) (AA 134-164), (Middle Region) antibody

Details for Product No. ABIN953376, Supplier: Login to see
Antigen
  • NY-REN-28
  • wu:fa14c11
  • zgc:86933
  • 1500012F11Rik
  • AU019660
  • AV213303
Epitope
AA 134-164, Middle Region
19
16
15
15
10
8
5
2
2
2
2
1
1
1
Reactivity
Human
70
9
8
2
2
2
Host
Rabbit
57
13
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
66
37
2
2
1
1
Supplier
Login to see
Supplier Product No.
Login to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen KLH conjugated synthetic peptide between 134-164 amino acids from the Central region of human MED6
Isotype Ig
Specificity This antibody recognizes Human MED6 (Center).
Purification Protein A column, followed by peptide affinity purification
Alternative Name MED6 (MED6 Antibody Abstract)
Background Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.Synonyms: ARC33, Activator-recruited cofactor 33 kDa component, Mediator complex 6, Mediator of RNA polymerase II transcription subunit 6, Renal carcinoma antigen NY-REN-28
Molecular Weight 28425 Da
Gene ID 10001
NCBI Accession NP_005457
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Mediator Complex Subunit 6 (MED6) (AA 134-164), (Middle Region) antibody (ABIN953376) Western blot analysis of MED6 Antibody (Center) in WiDr cell line lysates (35ug/lane)...
Background publications Pavri, Lewis, Kim et al.: "PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator." in: Molecular cell, Vol. 18, Issue 1, pp. 83-96, 2005 (PubMed).

Sato, Tomomori-Sato, Parmely et al.: "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." in: Molecular cell, Vol. 14, Issue 5, pp. 685-91, 2004 (PubMed).

Yang, DeBeaumont, Zhou et al.: "The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 8, pp. 2339-44, 2004 (PubMed).

Tomomori-Sato, Sato, Parmely et al.: "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5846-51, 2004 (PubMed).

Sato, Tomomori-Sato, Banks et al.: "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex." in: The Journal of biological chemistry, Vol. 278, Issue 50, pp. 49671-4, 2003 (PubMed).