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Rabbit anti-Goat IgG (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment
Catalog No. ABIN965040
  • Target See all IgG products
    IgG
    Fragment
    F(ab')2 fragment
    Binding Specificity
    • 2271
    • 457
    • 363
    • 188
    • 130
    • 123
    • 93
    • 29
    • 28
    • 28
    • 17
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 670
    • 659
    • 553
    • 424
    • 365
    • 219
    • 199
    • 144
    • 134
    • 122
    • 109
    • 93
    • 93
    • 91
    • 82
    • 58
    • 44
    • 28
    • 27
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Rabbit, Goat
    Host
    • 1649
    • 1417
    • 530
    • 214
    • 104
    • 90
    • 33
    • 30
    • 21
    • 16
    • 15
    • 11
    • 11
    • 1
    Rabbit
    Clonality
    • 3293
    • 191
    • 1
    Polyclonal
    Application
    • 1774
    • 1700
    • 1686
    • 1441
    • 934
    • 910
    • 883
    • 463
    • 234
    • 115
    • 115
    • 100
    • 92
    • 86
    • 77
    • 50
    • 48
    • 32
    • 31
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    F(ab')2 Anti-GOAT IgG (H&L) (RABBIT) Antibody assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.
    F(ab')2 anti-Goat IgG (H&L) antibody generated in rabbit detects specifically Goat IgG whole molecule. Antibody assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.
    Characteristics
    F(ab')2 anti-Goat IgG (H&L) antibody generated in rabbit detects specifically Goat IgG whole molecule. This secondary antibody anti-Goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Goat IgG whole molecule

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: F(ab')2 Anti-GOAT IgG (H&L) (RABBIT) Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Specific conditions should be optimized by researcher.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    DO NOT FREEZE. Dilute only prior to immediate use.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: Rabbit F(ab')2 Anti-Goat IgG Antibody, Rabbit Fab2 Anti-Goat IgG Antibody

    Background: F(ab')2 Anti-Goat IgG Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. F(ab')2 anti-Goat IgG antibody is ideal for investigators involved in Serum Protein Component research.

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