Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody

Details for Product No. ABIN965775
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Antigen
Synonyms MS4A1, bp35, cd20, ms4a2, leu-16, ms4a4c, cd20-like, B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7, AA960661, Cd20, Ly-44, Ms4a2
Reactivity
Human
(398), (47), (31), (30), (24), (20), (6), (6), (4), (2)
Host
Mouse
(296), (105), (8), (4), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(45), (26), (23), (16), (7), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)
(233), (102), (83), (60), (56), (54), (47), (22), (20), (12), (7), (3), (1), (1)
Pubmed 2 references available
Quantity 0.5 mL
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Catalog No. ABIN965775
192.50 $
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Immunogen Human leucocytes
Clone 109-3C2
Isotype IgG3
Specificity This antibody reacts with CD20, a 37/35kD non-glycosylated transmembrane phosphoprotein that is involved in the regulation of the actiavtion of B-cells.
Purification Concentrated.
Alternative Name CD20
Gene ID 931
Research Area Cancer, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Application Notes Immunohistochemistry: 1:50-1:100.
Flow cytometry: 1:150-1:300
Immunoprecipitation.
Staining Procedure: This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using with formalin-fixed paraffinembedded tissue sections an antigen demasking treatment is recommended. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Comment

Recommended Positive Control: Tonsil, Spleen, Thymus

Restrictions For Research Use only
Format Liquid
Concentration 0.3 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Einfeld, Brown, Valentine et al.: "Molecular cloning of the human B cell CD20 receptor predicts a hydrophobic protein with multiple transmembrane domains." in: The EMBO journal, Vol. 7, Issue 3, pp. 711-7, 1988 (PubMed).

Tedder, Klejman, Disteche et al.: "Cloning of a complementary DNA encoding a new mouse B lymphocyte differentiation antigen, homologous to the human B1 (CD20) antigen, and localization of the gene to chromosome 19." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 12, pp. 4388-94, 1989 (PubMed).

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