CHEK1 antibody

Catalog No. ABIN965882

There is 1 publication for this product available. The Monoclonal anti-CHEK1 antibody is suitable to detect CHEK1 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for CHEK1 antibody (ABIN965882)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human

Host: Please inquire

Clonality: Monoclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CHEK1 Antibody

Specificity: Ni-NTA purified recombinant protein CHK1 expressed in E. Coli strain M15.

Purification: Antibodies are purified by protein G affinity chromatography.

Isotype: IgG1

Application Details

Application Notes: Western Blot: Dilution 1: 2,00- 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References for anti-CHEK1 antibody (ABIN965882)

OConnell, Raleigh, Verkade, Nurse: "Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation." in: The EMBO journal, Vol. 16, Issue 3, pp. 545-54, (1997) (PubMed).

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHK1

Background: Active recombinant full length human CHK1 containing an Nterminal GST tag. This protein has an apparent molecular weight of ~82kDa. CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia.

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair