Fibronectin antibody

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Antigen
  • FN
  • cig
  • fibronectin
  • finc
  • lets
  • msf
  • hypothetical protein
  • fibronectin 1
  • fibronectin
  • fn1
Reactivity
Human
307
95
82
29
21
9
9
4
3
2
2
1
Host
Mouse
192
164
13
5
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
26
16
15
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro))
159
151
110
80
59
57
45
41
39
30
25
19
13
8
7
5
2
2
2
2
2
1
1
1
1
1
Options
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Immunogen T-cell lymphoma biopsy.
Clone B476
Isotype IgG1
Specificity This antibody reacts with fibronectin in the connective tissue matrix. This antibody does not react with plasma fibronectin.
Purification Concentrated.
Molecular Weight 440 kDa (non reduced), 220 kDa (reduced)
Gene ID 2335
Research Area Lineage Markers, Extracellular Matrix
Application Notes Immunohistochemistry: 1:50-1:100 on frozen tissue sections.
Staining Procedure: This antibody can be used on frozen tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Comment

Cellular Localization: Connective tissue and matrix.
Recommended Positive Control: Kidney (normal or cancerous)

Restrictions For Research Use only
Format Liquid
Concentration 0.3 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Background publications Martin, Buckwalter: "Effects of fibronectin on articular cartilage chondrocyte proteoglycan synthesis and response to insulin-like growth factor-I." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 16, Issue 6, pp. 752-7, 1999 (PubMed).

Joh, Speziale, Gurusiddappa, Manor, Höök: "Multiple specificities of the staphylococcal and streptococcal fibronectin-binding microbial surface components recognizing adhesive matrix molecules." in: European journal of biochemistry / FEBS, Vol. 258, Issue 2, pp. 897-905, 1999 (PubMed).

Peters, Hynes: "Fibronectin isoform distribution in the mouse. I. The alternatively spliced EIIIB, EIIIA, and V segments show widespread codistribution in the developing mouse embryo." in: Cell adhesion and communication, Vol. 4, Issue 2, pp. 103-25, 1997 (PubMed).

Ljubimov, Burgeson, Butkowski, Michael, Sun, Kenney: "Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 72, Issue 4, pp. 461-73, 1995 (PubMed).