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GST antibody (Glutathione S Transferase)

Details for Product anti-GST Antibody No. ABIN966238, Supplier: Log in to see
Antigen
  • GST
  • GST 13-13
  • GST13
  • GST13-13
  • GSTK1-1
  • hGSTK1
  • GSTS
  • GSTS1-1
  • PGD2
  • PGDS
  • H-PGDS
  • Ptgds2
  • 1500002K10Rik
  • Gst
Reactivity
Schistosoma japonicum
369
93
35
9
7
5
3
2
1
1
1
1
Host
233
173
97
5
4
3
Clonality
Monoclonal
Conjugate
This GST antibody is un-conjugated
33
32
28
15
8
7
6
5
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
415
228
118
110
43
27
17
16
12
11
8
8
8
7
7
6
5
3
2
2
2
1
1
1
1
1
Supplier
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Isotype IgG1
Specificity Ni-NTA purified recombinant GST expressed in E. Coli strain M15.
Purification Antibodies are purified by protein A affinity chromatography
Alternative Name Glutathione-S-Transferase (GST) (GST Antibody Abstract)
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
Application Notes Western Blot: Dilution 1: 2000- 5,000
ELISA: Proposed dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Storage -20 °C
Product cited in: Smith, Johnson: "Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase." in: Gene, Vol. 67, Issue 1, pp. 31-40, 1988 (PubMed).