Human WAF 1/p21 Protein antibody

Antigen: Human WAF 1/p21 Protein

Clonality: Monoclonal (B459)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN966329

Additional Information

Immunogen: Human p21 protein.

Isotype: IgG1

Clone: B459

Specificity: This antibody reacts with human and other mammalian p21 protein. p21 is an intracellular protein of 21kD size. It is also known as the wild type p53 activated fragment 1 or WAF1. p21 inhibits cyclin-dependent kinases (Cdk's) and also proliferating cell nuclear antigens or PCNA's).

Molecular Weight: 21 kDa

Comments:

Cellular Localization: Nuclear.
Recommended Positive Control: Breast, Skin, or Colon Carcinoma, Fibroadenoma.

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100 on tissue sections.
Western blotting: 1:100-1:300.
ELISA: 1:500-1:3000.
Staining Procedure: This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using on formalin-fixed paraffinembedded tissues a antigen demasking procedure is recommended.The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Concentration: 0.3 mg/ml.

Purification: Concentrated.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: 0.05% Sodium Azide

Storage: Store at 2-8° C for short term, freeze under -20°C for long term storage.

Restrictions: For Research Use only

Publications

Product: Nakanishi, Robetorye, Pereira-Smith et al.: "The C-terminal region of p21SDI1/WAF1/CIP1 is involved in proliferating cell nuclear antigen binding but does not appear to be required for growth inhibition." in: The Journal of biological chemistry, Vol. 270, Issue 29, pp. 17060-3, 1995 (PubMed).

Nakanishi, Robetorye, Adami et al.: "Identification of the active region of the DNA synthesis inhibitory gene p21Sdi1/CIP1/WAF1." in: The EMBO journal, Vol. 14, Issue 3, pp. 555-63, 1995 (PubMed).

el-Deiry, Tokino, Velculescu et al.: "WAF1, a potential mediator of p53 tumor suppression." in: Cell, Vol. 75, Issue 4, pp. 817-25, 1993 (PubMed).