MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5)

Details for Product anti-MAP3K5 Antibody No. ABIN966515, Supplier: Log in to see
Antigen
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAPKKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • MEKK5
  • MAP3K5
  • mitogen-activated protein kinase kinase kinase 5
  • Map3k5
  • MAPKKK5
  • MAP3K5
Alternatives
anti-Human MAP3K5 antibody for Western Blotting
Reactivity
Human
400
172
97
6
1
1
1
1
1
1
Host
Mouse
380
26
Clonality (Clone)
Monoclonal ()
Conjugate
This MAP3K5 antibody is un-conjugated
12
12
11
6
6
6
5
5
5
5
5
5
5
5
5
5
5
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
304
159
133
82
65
42
21
12
12
10
4
3
2
1
1
Options
Supplier
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Immunogen Purified recombinant fragment of human MAP3K5 expressed in E. Coli.
Clone 2E4
Isotype IgG1
Purification Ascitic fluid
Alternative Name MAP3K5 (MAP3K5 Antibody Abstract)
Background Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun Nterminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.
Synonyms: ASK1, MEKK5, MAPKKK5
Molecular Weight 55kDa
Gene ID 4217
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response
Application Notes Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Pressinotti, Klocker, Schäfer, Luu, Ruschhaupt, Kuner, Steiner, Poustka, Bartsch, Sültmann: "Differential expression of apoptotic genes PDIA3 and MAP3K5 distinguishes between low- and high-risk prostate cancer." in: Molecular cancer, Vol. 8, pp. 130, 2010 (PubMed).

Widenmaier, Ao, Kim, Warnock, McIntosh et al.: "Suppression of p38 MAPK and JNK via Akt-mediated inhibition of apoptosis signal-regulating kinase 1 constitutes a core component of the beta-cell pro-survival effects of glucose-dependent ..." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30372-82, 2009 (PubMed).

Woolcott, SenGupta, Hanna, Aronson: "Estrogen and progesterone receptor levels in nonneoplastic breast epithelium of breast cancer cases versus benign breast biopsy controls." in: BMC cancer, Vol. 8, pp. 130, 2008 (PubMed).

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