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Tumor Protein P53 (TP53) antibody

Antigen

Tumor Protein P53 (TP53)

Synonyms p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53
Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human

Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN966758
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information

Alternative name P53
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of serine15 (P-L-SP-Q-E).
Specificity P53 (Ab-15) antibody detects endogenous levels of total p53 protein.

Application Details

Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications

Product Wang, Wiltshire, Wang et al.: "ATM-dependent CHK2 activation induced by anticancer agent, irofulven." in: The Journal of biological chemistry, Vol. 279, Issue 38, pp. 39584-92, 2004 (PubMed).

Li, Zhang, Sejas et al.: "Hypoxia-induced nucleophosmin protects cell death through inhibition of p53." in: The Journal of biological chemistry, Vol. 279, Issue 40, pp. 41275-9, 2004 (PubMed).

Vega, Sevilla, Lazo: "p53 Stabilization and accumulation induced by human vaccinia-related kinase 1." in: Molecular and cellular biology, Vol. 24, Issue 23, pp. 10366-80, 2004 (PubMed).

Lin, Chao, Saito et al.: "p53 induces differentiation of mouse embryonic stem cells by suppressing Nanog expression." in: Nature cell biology, Vol. 7, Issue 2, pp. 165-71, 2005 (PubMed).

Alternatives

Alternatives for antigen "Tumor Protein P53 (TP53)", type "Antibodies"
Hosts Rabbit (680), Mouse (309), Sheep (4), Escherichia Coli (E. coli) (1), Rat (1), Recombinant (1)
Reactivities Human (937), Mouse (Murine) (251), Rat (Rattus) (206), Cow (Bovine) (121), Dog (Canine) (113), Horse (Equine) (113), Pig (Porcine) (95), Sheep (Ovine) (93), Monkey (42), Guinea Pig (36), Chicken (20), Primate (7), Hamster (6), Rabbit (6), Cat (Feline) (5), Xenopus laevis (3), Mammalian (2), Bird (Avian) (1)
Applications Western Blotting (WB) (613), Immunofluorescence (IF) (355), ELISA (288), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (266), Immunoprecipitation (IP) (177), Immunohistochemistry (IHC) (157), Flow Cytometry (FACS) (105), Immunocytochemistry (ICC) (60), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (52), Immunohistochemistry (Frozen Sections) (IHC (fro)) (51), Immunohistochemistry (Fixed) (IHC (fx)) (22), Immunoelectron Microscopy (IEM) (16), Dot Blot (Dot) (9), Chromatin Immunoprecipitation (ChiP) (8), Gel Shift (GS) (5), Radioimmunoassay (RIA) (4), Chromatin Immunoprecipitation (ChIP) (2), Affinity Purification (AP) (1), Cellular Assay (CA) (1), Dot Blot (DB) (1), ELISA (Capture) (1), ELISA (Detection) (1), Immunoassay (IA) (1), Immunostaining (ISt) (1), Transfected cell culture (TCC) (1)
Conjugates FITC (32), Biotin (27), HRP (18), PE (17), Alexa Fluor 350 (16), Alexa Fluor 488 (16), Alexa Fluor 555 (16), Alexa Fluor 647 (16), Cy3 (16), Cy5 (16), Cy5.5 (16), Cy7 (16), Gold (16), PE,Cy3 (14), PE,Cy5 (14), PE,Cy5.5 (14), PE,Cy7 (14), RPE (3), PE-Cy3 (2), PE-Cy5 (2), PE-Cy5.5 (2), PE-Cy7 (2)
Epitopes pSer315 (34), pSer37 (34), pSer46 (33), pSer15 (32), pSer33 (31), pSer392 (31), pThr18 (31), pSer6 (30), pSer20 (29), C-Term (28), AA 20-25 (27), pSer9 (26), N-Term (22), pSer376 (22), pThr55 (21), acLys382 (20), pThr81 (19), AA 371-380 (7), AA 10-16 (5), AA 16-25 (5), AA 26-35 (5), Ser37 (5), Ser46 (5), AA 14-389 (4), AA 56-65 (4), Ser315 (4), pSer378 (4), AA 181-190 (3), AA 213-217 (3), AA 32-79 (3), AA 35-45 (3), AA 46-53 (3), AA 46-55 (3), AA 88-109 (3), AA 16-30 (2), AA 306-393 (2), AA 378-393 (2), Ser15 (2), Ser33 (2), Ser6 (2), Thr18 (2), Thr55 (2), acLys317 (2), acLys379 (2), acLys386 (2), AA 1-12 (1), AA 14-289 (1), AA 15-40 (1), AA 156-214 (1), AA 20-31 (1), AA 361-382 (1), AA 367-381 (1), AA 37-45 (1), AA 37-5 (1), AA 383-393 (1), Internal Region (1), Mutant (1), Mutant,AA 213-217 (1), Ser15,Phosphospecific (1), Ser20 (1), Ser392 (1), Wild type,Mutant (1), Wild type,Mutant,AA 32-79 (1), Wild type,Mutant,AA 370-378 (1), Wildtype (1), acLys373 (1), pSer332 (1), pSer366 (1), pSer392,AA 378-393 (1), pSer63 (1), pSer95 (1), pThr387 (1), phospho (1)

Variants

Variants