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Tumor Protein P53 (TP53) antibody

Antigen

Tumor Protein P53 (TP53)
Synonyms p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN966759
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Alternative name P53
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of serine 315 (S-S-SP-P-Q).
Specificity P53 (Ab-315) antibody detects endogenous levels of total p53 protein.

Application Details
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Application Notes WB: 1:500~1:1000.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
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Product Lohrum, Scheidtmann: "Differential effects of phosphorylation of rat p53 on transactivation of promoters derived from different p53 responsive genes." in: Oncogene, Vol. 13, Issue 12, pp. 2527-39, 1997 (PubMed).

Lu, Fisher, Bailey et al.: "The CDK7-cycH-p36 complex of transcription factor IIH phosphorylates p53, enhancing its sequence-specific DNA binding activity in vitro." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5923-34, 1997 (PubMed).

Merrick, Zhou, Martin et al.: "Site-specific phosphorylation of human p53 protein determined by mass spectrometry." in: Biochemistry, Vol. 40, Issue 13, pp. 4053-66, 2001 (PubMed).

Alternatives
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Alternatives for antigen "Tumor Protein P53 (TP53)", type "Antibodies"
Hosts Rabbit (680), Mouse (309), Sheep (4), Escherichia Coli (E. coli) (1), Rat (1), Recombinant (1)
Reactivities Human (937), Mouse (Murine) (251), Rat (Rattus) (206), Cow (Bovine) (121), Dog (Canine) (113), Horse (Equine) (113), Pig (Porcine) (95), Sheep (Ovine) (93), Monkey (42), Guinea Pig (36), Chicken (20), Primate (7), Hamster (6), Rabbit (6), Cat (Feline) (5), Xenopus laevis (3), Mammalian (2), Bird (Avian) (1)
Applications Western Blotting (WB) (613), Immunofluorescence (IF) (355), ELISA (288), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (266), Immunoprecipitation (IP) (177), Immunohistochemistry (IHC) (157), Flow Cytometry (FACS) (105), Immunocytochemistry (ICC) (60), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (52), Immunohistochemistry (Frozen Sections) (IHC (fro)) (51), Immunohistochemistry (Fixed) (IHC (fx)) (22), Immunoelectron Microscopy (IEM) (16), Dot Blot (Dot) (9), Chromatin Immunoprecipitation (ChiP) (8), Gel Shift (GS) (5), Radioimmunoassay (RIA) (4), Chromatin Immunoprecipitation (ChIP) (2), Affinity Purification (AP) (1), Cellular Assay (CA) (1), Dot Blot (DB) (1), ELISA (Capture) (1), ELISA (Detection) (1), Immunoassay (IA) (1), Immunostaining (ISt) (1), Transfected cell culture (TCC) (1)
Conjugates FITC (32), Biotin (27), HRP (18), PE (17), Alexa Fluor 350 (16), Alexa Fluor 488 (16), Alexa Fluor 555 (16), Alexa Fluor 647 (16), Cy3 (16), Cy5 (16), Cy5.5 (16), Cy7 (16), Gold (16), PE,Cy3 (14), PE,Cy5 (14), PE,Cy5.5 (14), PE,Cy7 (14), RPE (3), PE-Cy3 (2), PE-Cy5 (2), PE-Cy5.5 (2), PE-Cy7 (2)
Epitopes pSer315 (34), pSer37 (34), pSer46 (33), pSer15 (32), pSer33 (31), pSer392 (31), pThr18 (31), pSer6 (30), pSer20 (29), C-Term (28), AA 20-25 (27), pSer9 (26), N-Term (22), pSer376 (22), pThr55 (21), acLys382 (20), pThr81 (19), AA 371-380 (7), AA 10-16 (5), AA 16-25 (5), AA 26-35 (5), Ser37 (5), Ser46 (5), AA 14-389 (4), AA 56-65 (4), Ser315 (4), pSer378 (4), AA 181-190 (3), AA 213-217 (3), AA 32-79 (3), AA 35-45 (3), AA 46-53 (3), AA 46-55 (3), AA 88-109 (3), AA 16-30 (2), AA 306-393 (2), AA 378-393 (2), Ser15 (2), Ser33 (2), Ser6 (2), Thr18 (2), Thr55 (2), acLys317 (2), acLys379 (2), acLys386 (2), AA 1-12 (1), AA 14-289 (1), AA 15-40 (1), AA 156-214 (1), AA 20-31 (1), AA 361-382 (1), AA 367-381 (1), AA 37-45 (1), AA 37-5 (1), AA 383-393 (1), Internal Region (1), Mutant (1), Mutant,AA 213-217 (1), Ser15,Phosphospecific (1), Ser20 (1), Ser392 (1), Wild type,Mutant (1), Wild type,Mutant,AA 32-79 (1), Wild type,Mutant,AA 370-378 (1), Wildtype (1), acLys373 (1), pSer332 (1), pSer366 (1), pSer392,AA 378-393 (1), pSer63 (1), pSer95 (1), pThr387 (1), phospho (1)

Variants
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Variants