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Keratin 7 (KRT7) antibody

Details for Product No. ABIN967289, Supplier: Login to see
Antigen
  • CK7
  • K2C7
  • K7
  • SCL
  • D15Wsu77e
  • Krt2-7
  • KRT7
Reactivity
Dog (Canine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
304
79
63
7
6
5
5
5
3
2
1
1
1
Host
Mouse
177
153
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
19
9
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
210
116
115
72
62
56
52
43
22
21
11
8
3
2
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Cytokeratins from the human bladder carcinoma cell line T24
Clone RCK105
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Hamster, Dog (Canine), Pig (Porcine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Cytokeratin 7 (KRT7 Antibody Abstract)
Background Intermediate filaments (IF) are a subset of cytoskeletal proteins which function to give overall structural integrity to the plasma membrane as well as organize cells into specific tissues. IF proteins can be divided into six major types based upon similarity in sequence. Type I and II IF proteins are designated keratins based upon whether they are acidic (I) or basic (II) and are characterized by their remarkable biochemical diversity. A subgroup of keratins, designated cytokeratins, are represented in epithelial tissues by at least 20 different polypeptides, designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin 7 is found in both columnar and glandular epithelium of the lung, cervix, breast, bile ducts, and in the collecting ducts in the kidney as well as mesothelium. The molecular weight of cytokeratin 7 is approximately 54 kDa in SDS/PAGE.
Molecular Weight 55 kDa
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Pathways
Application Notes Applications include western blot analysis. Additional applications include immunohistochemistry on methanol fixed tissue, but this application is not tested.
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-Keratin 7 (KRT7) antibody (ABIN967289) Western blot analysis of Cytokeratin 7. Lysate from HeLa cells was probed with anti-c...
Product cited in: Broers, Ramaekers, Rot et al.: "Cytokeratins in different types of human lung cancer as monitored by chain-specific monoclonal antibodies." in: Cancer research, Vol. 48, Issue 11, pp. 3221-9, 1988 (PubMed).

Ramaekers, Huysmans, Schaart et al.: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).

Smedts, Ramaekers, Robben et al.: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).