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GTPase Kras antibody

Details for Product No. ABIN967445
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Antigen
Synonyms
Kras2, c-Ki-ras, p21, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2, SIIR, pp21, 0610011M09Rik, BC011290, P21, AI929937, K-ras, Ki-ras, Kras-2, p21B, ras, wu: ... show more
Kras2, c-Ki-ras, p21, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2, SIIR, pp21, 0610011M09Rik, BC011290, P21, AI929937, K-ras, Ki-ras, Kras-2, p21B, ras, wu:fa04e08, wu:fc14b12, wu:fc23g10, wu:fj89d12, zgc:85725 show less
Reactivity
Human
(84), (39), (39), (13), (2), (1), (1), (1)
Host
Mouse
(73), (18), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe)), Immunoprecipitation (IP)
(70), (38), (24), (20), (11), (7), (6), (4), (2), (1)
Pubmed 8 references available
Quantity 0.1 mg
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Catalog No. ABIN967445
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Immunogen Human p21
Clone 6B6
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name p21
Background P21 belongs to a class of tumor suppressors including p16 and p27 which control progression through the cell cycle by inhibiting the activity of cyclin-cdk complexes. p21 is also known as senescent cell-derived inhibitor 1 (Sdi1), wild-type p53-activated fragment 1 (Waf1), Cdk-interacting protein 1 (Cip1), p21 and p53-regulated inhibitor of Cdks (Pic1). p21 mRNA is expressed at higher levels in senescent fibroblasts than in actively growing cells. When introduced into proliferating foreskin fibroblasts, p21 inhibits DNA synthesis and cell cycle arrest. p53 can activate p21 gene expression via a p53 binding site identified in the promoter of the p21 gene.
Molecular Weight 21 kDa
Research Area Cell Cycle
Application Notes Applications include immunoprecipitation (1-2 µg/1x10^6 cells), western blot analysis (1-2 µg/ml) and immunohistochemistry of paraformaldehyde-fixed cultured cells (0.5-2.5 µg/ml) and acetone-fixed, frozen and formalin-fixed paraffin-embedded (10 µg/ml) tissue sections. MCF7 human breast carcinoma cells (ATCC HTB-22) are suggested as a positive control. WI-38 human lung fibroblasts (ATCC CCL-75) treated with doxorubin (Adriamycin) can also be used as a positive control.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-GTPase Kras antibody Western blot analysis of p21. Lysate from MCF7 breast carcinoma cells was probed with anti-human p21.
anti-GTPase Kras antibody (2) anti-GTPase Kras antibody (Image 2)
General Clarke, Lotz, Chao et al.: "Activation of the p21 pathway of growth arrest and apoptosis by the beta 4 integrin cytoplasmic domain." in: The Journal of biological chemistry, Vol. 270, Issue 39, pp. 22673-6, 1995 (PubMed).

Zhang, Grasso, McClain et al.: "p53-independent induction of WAF1/CIP1 in human leukemia cells is correlated with growth arrest accompanying monocyte/macrophage differentiation." in: Cancer research, Vol. 55, Issue 3, pp. 668-74, 1995 (PubMed).

Halevy, Novitch, Spicer et al.: "Correlation of terminal cell cycle arrest of skeletal muscle with induction of p21 by MyoD." in: Science (New York, N.Y.), Vol. 267, Issue 5200, pp. 1018-21, 1995 (PubMed).

el-Deiry, Harper, OConnor et al.: "WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis." in: Cancer research, Vol. 54, Issue 5, pp. 1169-74, 1994 (PubMed).

Noda, Ning, Venable et al.: "Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen." in: Experimental cell research, Vol. 211, Issue 1, pp. 90-8, 1994 (PubMed).

el-Deiry, Tokino, Velculescu et al.: "WAF1, a potential mediator of p53 tumor suppression." in: Cell, Vol. 75, Issue 4, pp. 817-25, 1993 (PubMed).

Hunter: "Braking the cycle." in: Cell, Vol. 75, Issue 5, pp. 839-41, 1994 (PubMed).

Xiong, Hannon, Zhang et al.: "p21 is a universal inhibitor of cyclin kinases." in: Nature, Vol. 366, Issue 6456, pp. 701-4, 1994 (PubMed).

Validation Images
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