serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5) antibody

Details for Product No. ABIN967456
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Antigen
Synonyms 1110036M19Rik, AI462524, AI646751, Maspin, PI-5, Spi7, ovalbumin, PI5, maspin, Pi5
Reactivity
Human
(76), (32), (29), (1), (1), (1), (1), (1)
Host
Mouse
(59), (16), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(55), (38), (18), (17), (10), (9), (5), (2), (2), (1), (1)
Pubmed 3 references available
Catalog no. ABIN967456
Quantity 0.1 mg
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Immunogen Recombinant Human Maspin
Clone G167-70
Isotype IgG2b
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Maspin
Background Maspin is a 42 kDa protein that may play a role in human breast cancer by functioning as a tumor suppressor. Maspin is expressed in normal mammary epithelial cells, but there is a loss of expression in invasive breast cancer including lymph node and distant metastases. Further, maspin is not expressed in most mammary carcinoma cell lines. Mammary carcinoma cells transfected with recombinant maspin have a reduced ability to migrate through reconstituted basement membrane and lose their ability to form tumors in nude mice, providing support for maspin as a tumor suppressor protein. Maspin has homology to members of the serpin family of protease inhibitors, some of which have been implicated in the regulation of invasion and metastasis. Yet protease inhibitory activity has not been detected using recombinant maspin. It has been suggested that maspin, unlike most serpins, may be a ligand-binding serpin rather than a protease inhibitor. However, the question of whether or not maspin has protease inhibitor activity remains to be determined. G167-70 is specific for human maspin. It detects maspin as a 42 kD protein in western blots of normal human mammary epithelial cells. Many mammary tumor cell lines including MDA-MB-231 (ATCC HTB 26) and MDA-MB-435S (ATCC HTB 129) lack maspin expression and are suitable as negative controls. Purified recombinant human maspin from a yeast expression system was used as immunogen.
Molecular Weight 42 kDa
Application Notes Applications include western blot analysis (1-2 µg/ml). A-431 human epidermal carcinoma cells (ATCC CRL 1555) are recommended as a positive control for this application.
Comment

Related Products: ABIN967389, ABIN968533

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5) antibody Western blot analysis of maspin in A- 431 human epidermal carcinoma cell lysates. The G167-70 antibody was titrated between 1 µg/ml to 0.04 µg/ml (lanes 1-3) and identifies maspin at ~ 42 kD.
anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5) antibody (2) anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5) antibody (Image 2)
General Sheng, Pemberton, Sager: "Production, purification, and characterization of recombinant maspin proteins." in: The Journal of biological chemistry, Vol. 269, Issue 49, pp. 30988-93, 1995 (PubMed).

Hopkins, Whisstock: "Function of maspin." in: Science (New York, N.Y.), Vol. 265, Issue 5180, pp. 1893-4, 1994 (PubMed).

Zou, Anisowicz, Hendrix et al.: "Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells." in: Science (New York, N.Y.), Vol. 263, Issue 5146, pp. 526-9, 1994 (PubMed).

Hosts (59), (16), (1)
Reactivities (76), (32), (29), (1), (1), (1), (1), (1)
Applications (55), (38), (18), (17), (10), (9), (5), (2), (2), (1), (1)
Conjugates (6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (5), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1)
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