Nestin antibody (NES)

Details for Product anti-NES Antibody No. ABIN967492, Supplier: Log in to see
Antigen
  • paranemin
  • NES
  • AA166324
  • C78523
  • ESTM46
  • nestin
  • NES
  • nes
  • Nes
Reactivity
Rat (Rattus)
278
76
66
17
4
4
3
3
2
2
2
2
1
1
1
Host
Mouse
179
134
10
6
2
Clonality (Clone)
Monoclonal ()
Conjugate
This Nestin antibody is un-conjugated
13
13
13
11
6
6
6
6
6
5
5
5
5
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Fluorescence Microscopy (FM), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
227
110
107
104
104
94
84
47
15
13
11
5
4
2
1
1
Options
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Brand BD Pharmingen™
Immunogen Rat (E15) spinal cord extracts
Clone Rat 401
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Nestin (NES Antibody Abstract)
Background Multipotential stem cells in the neural tube of the developing embryo give rise to the different neuronal cell types of the brain. Nestin is an intermediate filament protein which is abundantly expressed in neuroepithelial stem cells early in embryogenesis, but is absent from nearly all mature central nervous system (CNS) cells. After its down-regulation, GFAP and neurofilaments are expressed in differentiated astrocytes and neurons, respectively. Initial studies showed that the mouse anti-rat nestin antibody (clone Rat 401) recognized transient radial glial cells and dividing neuroepithelial stem cells in the embryonic rat CNS. Reportedly, Rat 401 has been used to analyze nestin expression in the developing rat nervous system and in immortalized CNS precursor cell lines (e.g such as in E11 rat CNS stem cells, but lost by postnatal day 6 (P6) in spinal cord and by P21 in the cerebellum). Although not expressed in normal adult CNS cells, nestin has been reportedly to be detectable in a variety of CNS tumors, suggesting that these tumors share gene expression patterns with primitive, undifferentiated CNS cells. Rat 401 positive cells have been reported to be found throughout the developing neural tube, but not in the adult CNS. More than 90% of dissociated E11 neural tube cells have been reported to be Rat 401 positive. Rat 401 recognizes rat nestin and has been reported not to cross-react with human nestin. Due to differential tissue expression, this antibody may recognize nestin as a doublet within a range of 198-260 kD.
Molecular Weight 198-260 kDa
Research Area Angiogenesis, Lineage Markers
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-Nestin antibody (NES) (ABIN967492) Western blot analysis of Nestin. A rat (E21) cerebrum lysate was probed with the mous...
 image for anti-Nestin antibody (NES) (ABIN967492) anti-Nestin (NES) antibody (Image 2)
Western Blotting (WB) image for anti-Nestin antibody (NES) (ABIN967492) anti-Nestin (NES) antibody (Image 3)
Product cited in: Dahlstrand, Zimmerman, McKay, Lendahl: "Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments." in: Journal of cell science, Vol. 103 ( Pt 2), Issue 1, pp. 589-97, 1993 (PubMed).

Tohyama, Lee, Rorke, Marvin, McKay, Trojanowski: "Monoclonal antibodies to a rat nestin fusion protein recognize a 220-kDa polypeptide in subsets of fetal and adult human central nervous system neurons and in primitive neuroectodermal tumor cells." in: The American journal of pathology, Vol. 143, Issue 1, pp. 258-68, 1993 (PubMed).

Dahlstrand, Collins, Lendahl: "Expression of the class VI intermediate filament nestin in human central nervous system tumors." in: Cancer research, Vol. 52, Issue 19, pp. 5334-41, 1992 (PubMed).

Tohyama, Lee, Rorke, Marvin, McKay, Trojanowski: "Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 66, Issue 3, pp. 303-13, 1992 (PubMed).

Renfranz, Cunningham, McKay: "Region-specific differentiation of the hippocampal stem cell line HiB5 upon implantation into the developing mammalian brain." in: Cell, Vol. 66, Issue 4, pp. 713-29, 1991 (PubMed).

Lendahl, Zimmerman, McKay: "CNS stem cells express a new class of intermediate filament protein." in: Cell, Vol. 60, Issue 4, pp. 585-95, 1990 (PubMed).

Geschwind, Hockfield: "Identification of proteins that are developmentally regulated during early cerebral corticogenesis in the rat." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 9, Issue 12, pp. 4303-17, 1990 (PubMed).

Frederiksen, McKay: "Proliferation and differentiation of rat neuroepithelial precursor cells in vivo." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 4, pp. 1144-51, 1988 (PubMed).

Hockfield, McKay: "Identification of major cell classes in the developing mammalian nervous system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 5, Issue 12, pp. 3310-28, 1986 (PubMed).