SRSF2 antibody (serine/arginine-Rich Splicing Factor 2)

Details for Product anti-SRSF2 Antibody No. ABIN967516, Supplier: Log in to see
Antigen
  • PR264
  • SC-35
  • SC35
  • SFRS2
  • SFRS2A
  • SRp30b
  • D11Wsu175e
  • MRF-1
  • Pr264
  • Sfrs10
  • Sfrs2
  • pr264
  • sc-35
  • sc35
  • sfrs2
  • sfrs2a
  • srp30b
  • serine/arginine-rich splicing factor 2
  • SRSF2
  • Srsf2
  • srsf2
Reactivity
Human
33
12
9
4
3
3
3
3
2
2
2
2
2
Host
Mouse
29
4
Clonality (Clone)
Monoclonal ()
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
28
12
6
5
5
5
4
2
2
1
Options
Supplier
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Supplier Product No.
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Brand BD Pharmingen™
Immunogen Partially purified spliceosomes
Clone AlphaSC35
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name SC35 (SRSF2 Antibody Abstract)
Background SC35 belongs to a highly conserved SR splicing factor family. Members of this family have characteristic RNA-binding domains and serine/arginine-rich (SR) motifs. SR proteins are expressed in the nucleus and are essential pre-messenger RNA splicing factors. Nuclear pre-messenger RNA takes place in spliceosomes, multicomponent complexes consisting of five small nuclear ribonucleoprotein particles (U1, U2, U4, U5, U6) and other non-snRNP factors such as SC35. SC35 is necessary for an early step in spliceosome assembly and can also influence the selection of alternative splice sites. SC35 is identified as a 35 kDa protein in SDS/PAGE. Clone alphaSC35 has been shown to recognize SC35 in human, and rat cells. However, it should recognize SC35 from any species, as SC35 is conserved from Drosophila to man. Partially purified spliceosomes were used as immunogen.
Research Area DNA/RNA
Application Notes Staining is nuclear, a punctate or speckled nuclear pattern may be detected. Only a subset of cells are stained, the percentage may vary between cell type and experimental conditions. MG-63 human osteosarcoma, HeLa carcinoma (ATCC CCL-2), or BRL-3A rat liver cells (ATCC CRL-1442) are suggested as positive controls.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-serine/arginine-Rich Splicing Factor 2 (SRSF2) antibody (ABIN967516) Western blot analysis of SC35. Lysates from MG-63 human osteosarcoma cells were probe...
 image for anti-serine/arginine-Rich Splicing Factor 2 (SRSF2) antibody (ABIN967516) anti-serine/arginine-Rich Splicing Factor 2 (SRSF2) antibody (Image 2)
Product cited in: Sukegawa, Blobel: "A putative mammalian RNA helicase with an arginine-serine-rich domain colocalizes with a splicing factor." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15702-6, 1995 (PubMed).

Fu: "Specific commitment of different pre-mRNAs to splicing by single SR proteins." in: Nature, Vol. 365, Issue 6441, pp. 82-5, 1993 (PubMed).

Fu, Maniatis: "Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus." in: Nature, Vol. 343, Issue 6257, pp. 437-41, 1990 (PubMed).

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