VCAM1 antibody

Catalog No. ABIN967640

Product Details anti-VCAM1 Antibody

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This VCAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat VCAM-1-transfected L5178Y Cells

Clone: MR106

Isotype: IgG1 kappa

Application Details

Application Notes: Caution: Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-VCAM1 antibody (ABIN967640)

Bevilacqua: "Endothelial-leukocyte adhesion molecules." in: Annual review of immunology, Vol. 11, Issue 4, pp. 767-804, (1993) (PubMed).

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: CD106 (VCAM1 Products)

Synonyms: CD106 antibody, INCAM-100 antibody, Vcam-1 antibody, VCAM1B antibody, fi76d06 antibody, wu:fi76d06 antibody, zgc:158875 antibody, VCAM1 antibody, vascular cell adhesion molecule 1 antibody, vascular cell adhesion molecule 1b antibody, VCAM1 antibody, Vcam1 antibody, vcam1b antibody, VCAM-1 antibody

Background: The MR106 antibody reacts with CD106, the ~110 kDa transmembrane vascular cell adhesion molecule-1 (VCAM-1). Expression of CD106 on endothelial cells is induced by inflammatory cytokines and in certain pathologic conditions. In the rat bone marrow, CD106 has been reported to be expressed on dendritic cells and subsets of the myeloid lineage, but not detectable on peripheral leukocytes. CD106 has been reported to be a counter-receptor for VLA-4 (alpha4beta1 integrin) and alpha4beta7 integrin, and it plays an important role in leukocyte extravasation, particularly at sites of inflammation. This antibody is routinely tested by flow cytometric analysis.
Synonyms: VCAM-1

Pathways: Carbohydrate Homeostasis