V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 183-199) antibody

Antigen: V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF)

Synonyms: PKS2, A-RAF, ARAF1, RAFA1, zgc:92074, wu:fk45h07, ARAF, MGC128775, a-raf, MGC68526, araf, pks2, araf1, rafa1

Binding Site: AA 183-199

Clonality: Monoclonal (1)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN967727

Additional Information

Alternative name: A-Raf

Immunogen: Mouse A-Raf

Cross-Reactivity: Human, Chicken, Dog (Canine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 1

Description: A-Raf, a proto-oncogene that encodes a 68 kDa serine/threonine-specific protein kinase, shows strong homology to c-Raf and v-Raf in the kinase region of the molecule. c-Raf appears to be uniformly and ubiquitously expressed, but A-Raf expression is much more variable. A-Raf is found at highest levels in urogenital and kidney tissues, with the lowest levels found in brain. Cell transformation by A-Raf has been shown to be independent of p21[ras], suggesting the possibility of an independent signal transduction pathway. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 68 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of A-Raf on SKN, a human neuroblastoma, cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- A-Raf antibody.

Immunoflourescence staining of rabbit kidney.

anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 183-199) antibody (Image 3)

Publications

Raabe, Rapp: "KSR--a regulator and scaffold protein of the MAPK pathway." in: Science's STKE : signal transduction knowledge environment, Vol. 2002, Issue 136, pp. pe28, 2002 (PubMed).

Product: Storm, Cleveland, Rapp: "Expression of raf family proto-oncogenes in normal mouse tissues." in: Oncogene, Vol. 5, Issue 3, pp. 345-51, 1990 (PubMed).

Rapp, Heidecker, Huleihel et al.: "raf family serine/threonine protein kinases in mitogen signal transduction." in: Cold Spring Harbor symposia on quantitative biology, Vol. 53 Pt 1, pp. 173-84, 1989 (PubMed).

Huleihel, Goldsborough, Cleveland et al.: "Characterization of murine A-raf, a new oncogene related to the v-raf oncogene." in: Molecular and cellular biology, Vol. 6, Issue 7, pp. 2655-62, 1987 (PubMed).

Gomez, Pritchard, Herbert: "cAMP-dependent protein kinase and Ca2+ influx through L-type voltage-gated calcium channels mediate Raf-independent activation of extracellular regulated kinase in response to glucagon-like peptide-1 in pancreatic beta-cells." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48146-51, 2002 (PubMed).