V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 183-199) antibody
|Synonyms||PKS2, A-RAF, ARAF1, RAFA1, zgc:92074, wu:fk45h07, ARAF, MGC128775, a-raf, MGC68526, araf, pks2, araf1, rafa1|
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
|5 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
|Cross-Reactivity||Human, Chicken, Dog (Canine), Rat (Rattus)|
|Description||A-Raf, a proto-oncogene that encodes a 68 kDa serine/threonine-specific protein kinase, shows strong homology to c-Raf and v-Raf in the kinase region of the molecule. c-Raf appears to be uniformly and ubiquitously expressed, but A-Raf expression is much more variable. A-Raf is found at highest levels in urogenital and kidney tissues, with the lowest levels found in brain. Cell transformation by A-Raf has been shown to be independent of p21[ras], suggesting the possibility of an independent signal transduction pathway. This antibody is routinely tested by western blot analysis.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||68 kDa|
Related Products: ABIN968533, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
Raabe, Rapp: "KSR--a regulator and scaffold protein of the MAPK pathway." in: Science's STKE : signal transduction knowledge environment, Vol. 2002, Issue 136, pp. pe28, 2002 (PubMed).
Storm, Cleveland, Rapp: "Expression of raf family proto-oncogenes in normal mouse tissues." in: Oncogene, Vol. 5, Issue 3, pp. 345-51, 1990 (PubMed).
Rapp, Heidecker, Huleihel et al.: "raf family serine/threonine protein kinases in mitogen signal transduction." in: Cold Spring Harbor symposia on quantitative biology, Vol. 53 Pt 1, pp. 173-84, 1989 (PubMed).
Huleihel, Goldsborough, Cleveland et al.: "Characterization of murine A-raf, a new oncogene related to the v-raf oncogene." in: Molecular and cellular biology, Vol. 6, Issue 7, pp. 2655-62, 1987 (PubMed).
Gomez, Pritchard, Herbert: "cAMP-dependent protein kinase and Ca2+ influx through L-type voltage-gated calcium channels mediate Raf-independent activation of extracellular regulated kinase in response to glucagon-like peptide-1 in pancreatic beta-cells." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48146-51, 2002 (PubMed).
|Hosts||Rabbit (43), Mouse (1)|
|Reactivities||Human (43), Rat (Rattus) (37), Mouse (Murine) (36), Cow (Bovine) (16), Dog (Canine) (16), Pig (Porcine) (16), Horse (Equine) (15), Sheep (Ovine) (10)|
|Applications||Immunofluorescence (IF) (31), Western Blotting (WB) (12), ELISA (11), Immunohistochemistry (IHC) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)|
|Epitopes||pSer214 (4), pSer299 (4), pTyr302,pTyr301 (4), Internal Region (2), AA 151-251 (1), Center (1), pSer255,pSer250,pSer245 (1), pSer595 (1), pSer645 (1), pTyr302 (1)|