STAT5A antibody (AA 451-649)

Catalog No. ABIN967810

This Mouse Monoclonal antibody specifically detects STAT5A in WB, IHC, IF and IP. It exhibits reactivity toward Human, Mouse, Rat and Dog and has been mentioned in 5+ publications.

Quick Overview for STAT5A antibody (AA 451-649) (ABIN967810)

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 89-Stat5

Product Details anti-STAT5A Antibody

Binding Specificity: AA 451-649

Cross-Reactivity: Human, Dog (Canine), Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Sheep Stat5 aa. 451-649

Isotype: IgG2b

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-STAT5A antibody (ABIN967810)

Buitenhuis, Baltus, Lammers, Coffer, Koenderman: "Signal transducer and activator of transcription 5a (STAT5a) is required for eosinophil differentiation of human cord blood-derived CD34+ cells." in: Blood, Vol. 101, Issue 1, pp. 134-42, (2002) (PubMed).

Chughtai, Schimchowitsch, Lebrun, Ali: "Prolactin induces SHP-2 association with Stat5, nuclear translocation, and binding to the beta-casein gene promoter in mammary cells." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 31107-14, (2002) (PubMed).

Sadowski, Choi, Le, Wheeler, Wang, Sadowski: "Insulin Induction of SOCS-2 and SOCS-3 mRNA expression in C2C12 Skeletal Muscle Cells Is Mediated by Stat5*." in: The Journal of biological chemistry, Vol. 276, Issue 23, pp. 20703-10, (2001) (PubMed).

Kirken, Malabarba, Xu, Liu, Farrar, Hennighausen, Larner, Grimley, Rui: "Prolactin stimulates serine/tyrosine phosphorylation and formation of heterocomplexes of multiple Stat5 isoforms in Nb2 lymphocytes." in: The Journal of biological chemistry, Vol. 272, Issue 22, pp. 14098-103, (1997) (PubMed).

Gouilleux, Wakao, Mundt, Groner: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, (1994) (PubMed).

Target Details for STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Alternative Name: Stat5

Background: Stat5, also known as mammary gland factor (MGF) has been shown to play a critical role in the lactogenic hormone response and has been well characterized in mammary epithelial cells. The SH3 domain of MGF shows extensive homology with the SH2 domains of Stat1 and Stat2. This homology has led to its classification as a member of the Stat family. The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment and activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Stat5 activity is tightly regulated throughout gestation, lactation, and post-lactation.
Synonyms: MGF

Molecular Weight: 92 kDa

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation