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Ras-related Protein Ral-A (rala) (AA 35-206) antibody

Details for Product No. ABIN967836, Supplier: Login to see
Antigen
  • 3010001O15Rik
  • AW322615
  • Ral
  • Rasl1
  • RAL
Alternatives
anti-Rat (Rattus) Ras-related Protein Ral-A antibody for Immunohistochemistry
Epitope
AA 35-206
16
16
9
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Rat (Rattus)
57
12
10
1
1
Host
Mouse
46
9
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
2
2
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
49
27
5
3
1
1
1
1
1
1
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Immunogen Human Ral A aa. 35-206
Clone 8-Ral A
Isotype IgG2a, kappa
Cross-Reactivity Rat (Rattus), Chicken, Dog (Canine), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Ral A (rala Antibody Abstract)
Background Ral is a low molecular weight GTP-binding protein belonging to the Ras superfamily of GTP-binding proteins and shows 50% amino acid identity to Ras. Ral cDNA clones have been isolated from human placenta, human pheochromocytoma, simian ß-lymphocyte, and marine ray electric lobe cDNA libraries. In humans, cDNA sequences for Ral A and RalB have been determined. The predicted amino acid sequences show 85% identity. Both proteins consist of 206 amino acids with a predicted molecular weight of 24kDa. However, Ral A isolated from human platelets shows an apparent molecular weight of 26-28kDa. Ral A mRNA levels in adult mouse tissues appear to be highest in testes, ovary, and brain, with lower levels found in the liver, spleen, kidney, thymus, heart, and salivary glands. Similar results have been observed in rat tissues. This antibody is routinely tested by western blot analysis.
Molecular Weight 24-28 kDa
Pathways Neurotrophin Signaling Pathway
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Ras-related Protein Ral-A (rala) (AA 35-206) antibody (ABIN967836) Western blot analysis of Ral A on a rat cerebrum lysate. Lane 1: 1:5000, lane 2: 1:10...
Immunofluorescence (IF) image for anti-Ras-related Protein Ral-A (rala) (AA 35-206) antibody (ABIN967836) Immunofluorescence staining of rat neurons at 5 µg/ml of the anti-Ral A antibody.
 image for anti-Ras-related Protein Ral-A (rala) (AA 35-206) antibody (ABIN967836) anti-Ras-related Protein Ral-A (rala) (AA 35-206) antibody (Image 3)
Western Blotting (WB) image for anti-Ras-related Protein Ral-A (rala) (AA 35-206) antibody (ABIN967836) anti-Ras-related Protein Ral-A (rala) (AA 35-206) antibody (Image 4)
Product cited in: Xu, Frankel, Jackson et al.: "Elevated phospholipase D activity in H-Ras- but not K-Ras-transformed cells by the synergistic action of RalA and ARF6." in: Molecular and cellular biology, Vol. 23, Issue 2, pp. 645-54, 2003 (PubMed).

Kinashi, Katagiri, Watanabe et al.: "Distinct mechanisms of alpha 5beta 1 integrin activation by Ha-Ras and R-Ras." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 22590-6, 2000 (PubMed).

Goi, Rusanescu, Urano et al.: "Ral-specific guanine nucleotide exchange factor activity opposes other Ras effectors in PC12 cells by inhibiting neurite outgrowth." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 1731-41, 1999 (PubMed).

Polakis, Weber, Nevins et al.: "Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets." in: The Journal of biological chemistry, Vol. 264, Issue 28, pp. 16383-9, 1989 (PubMed).

Olofsson, Chardin, Touchot et al.: "Expression of the ras-related ralA, rho12 and rab genes in adult mouse tissues." in: Oncogene, Vol. 3, Issue 2, pp. 231-4, 1988 (PubMed).