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MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (AA 1-110)

Details for Product anti-MAP2K2 Antibody No. ABIN967846, Supplier: Log in to see
Antigen
  • AA589381
  • ATMKK2
  • CFC4
  • F27B13.50
  • F27B13_50
  • MAP KINASE KINASE 1
  • MAP kinase kinase 2
  • MAPKK2
  • MEK2
  • MK1
  • MK2
  • MKK2
  • PRKMK2
  • Prkmk2
Alternatives
anti-Human MAP2K2 antibody for Western Blotting
Epitope
AA 1-110
34
23
15
15
15
14
14
11
10
8
8
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
243
110
93
8
5
2
2
1
Host
Mouse
197
43
9
1
Clonality (Clone)
Monoclonal ()
Conjugate
This MAP2K2 antibody is un-conjugated
10
10
9
7
7
7
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
200
86
81
43
33
27
23
15
15
11
11
4
1
1
1
Supplier
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Immunogen Rat MEK2 aa. 1-110
Clone 96-MEK2
Isotype IgG2a
Cross-Reactivity Mouse (Murine), Chicken, Dog (Canine), Frog, Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name MEK2 (MAP2K2 Antibody Abstract)
Background MEK2 (MAP Kinase Kinase 2 or ERK Kinase), a 46 kDa protein kinase, phosphorylates MAP kinases (ERKs) at tyrosine and threonine residues. This phosphorylation results in activation of the MAP kinases. MEK2 is seven amino acids larger and shares 81% identity with MEK1. In cultured cells, MEK2 is activated by serum. In vitro, v-Raf phosphorylates and activates MEK2. It is thought that all of these activated protein kinases are downstream of the Ras signal transduction pathway and represent an integral part of the Ras mitogenic signal. This antibody is routinely tested by western blot analysis.
Synonyms: MAP Kinase Kinase 2, ERK Kinase
Molecular Weight 46 kDa
Research Area Cancer, Tyrosine Kinases, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (AA 1-110) (ABIN967846) Western blot analysis of MEK2 on a RSV-3T3 cell lysate. Lane 1: 1:2500, lane 2: 1:500...
Immunofluorescence (IF) image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (AA 1-110) (ABIN967846) Immunofluorescence staining of human fibroblasts.
 image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (AA 1-110) (ABIN967846) anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-110) antibody (Image 3)
Western Blotting (WB) image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (AA 1-110) (ABIN967846) anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-110) antibody (Image 4)
Product cited in: Tworkowski, Salghetti, Tansey: "Stable and unstable pools of Myc protein exist in human cells." in: Oncogene, Vol. 21, Issue 55, pp. 8515-20, 2002 (PubMed).

Downey, Butler, Tapper, Fialkow, Saltiel, Rubin, Grinstein: "Importance of MEK in neutrophil microbicidal responsiveness." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 1, pp. 434-43, 1998 (PubMed).

Wu, Harrison, Dent, Lynch, Weber, Sturgill: "Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4539-48, 1993 (PubMed).

Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Hattori, Fukuda, Yamashita, Nakamura, Gotoh, Nishida: "Activation of mitogen-activated protein kinase and its activator by ras in intact cells and in a cell-free system." in: The Journal of biological chemistry, Vol. 267, Issue 28, pp. 20346-51, 1992 (PubMed).