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ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (AA 468-619)

Details for Product anti-ZAP70 Antibody No. ABIN967849, Supplier: Login to see New
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Synonyms zap70, MGC80670, MGC88962, ZAP70, 70kDa, AI327364, Srk, TZK, ZAP-70, mrtle, mur, SRK, STCD, STD
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AA 468-619
(53), (51), (47), (29), (22), (22), (22), (21), (15), (15), (15), (14), (13), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Chicken, Human, Mouse (Murine), Rat (Rattus)
(484), (170), (118), (4), (3), (3), (2), (2), (2)
(316), (149), (15), (8), (2)
Clonality (Clone)
Monoclonal ()
This ZAP70 antibody is un-conjugated
(31), (22), (17), (14), (10), (7), (5), (5), (4), (4), (4), (4), (4), (4), (4), (1)
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(297), (166), (156), (90), (72), (46), (44), (44), (15), (7), (5), (4), (4), (3), (2)
Pubmed 5 references available
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Quantity 150 μg
Shipping to United States ( )
Immunogen Human ZAP-70 Kinase aa. 468-619
Clone 29-ZAP70 Kinase
Isotype IgG2a
Cross-Reactivity Mouse (Murine), Rat (Rattus), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background ZAP-70 is a protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 contains two SH2-like domains with the PTK domain being at the C-terminus. It appears that both ZAP-70 and Syk are recruited to the phosphorylated CD3 and zeta subunits after TCR stimulation. The src-family PTKs, lck, and fyn, seem to play a role upstream of ZAP-70 and Syk where they phosphorylate CD3 and zeta. Thus both Src- and ZAP-70/Syk-family PTKs are critical for efficient induction of tyrosine phosphoproteins in heterologous cells. The significance of ZAP-70 in mediating TCR signal transduction has been confirmed by showing that ZAP-70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP-70 kinase domain which affect the stability of the protein. The levels of lck and fyn appear to be normal in the same cells. Results of the ZAP-70 mutations in these patients include an unusual absence of peripheral CD8+ T cells and an abundance of CD4+ T cells in peripheral lymphoid organs. These results indicate that these two populations of T cells develop through utilization of different intracellular signaling pathways.
Molecular Weight 70 kDa
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (AA 468-619) (ABIN967849) Western blot analysis of ZAP-70 Kinase on a Jurkat cell lysate (Human T-cell leukemia...
 image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (AA 468-619) (ABIN967849) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 468-619) antibody (Image 2)
Western Blotting (WB) image for anti-ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (AA 468-619) (ABIN967849) anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 468-619) antibody (Image 3)
Product cited in: Michel, Attal-Bonnefoy, Mangino et al.: "CD28 as a molecular amplifier extending TCR ligation and signaling capabilities." in: Immunity, Vol. 15, Issue 6, pp. 935-45, 2001 (PubMed).

Mora, Stanley, Armistead et al.: "Inefficient ZAP-70 phosphorylation and decreased thymic selection in vivo result from inhibition of NF-kappaB/Rel." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5628-35, 2001 (PubMed).

Maccalli, Pisarra, Vegetti et al.: "Differential loss of T cell signaling molecules in metastatic melanoma patients' T lymphocyte subsets expressing distinct TCR variable regions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6912-23, 2000 (PubMed).

Chan, Kadlecek, Elder et al.: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994 (PubMed).

Arpaia, Shahar, Dadi et al.: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, 1994 (PubMed).

Catalog No. ABIN967849
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