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Nitric Oxide Synthase 1, Neuronal (NOS1) (AA 1095-1289) antibody

Details for Product No. ABIN967895, Supplier: Log in to see
Antigen
  • nos
  • nNOS
  • NOS1
  • IHPS1
  • N-NOS
  • NC-NOS
  • NOS
  • bNOS
  • 2310005C01Rik
  • NO
  • NOS-I
  • Nos-1
Epitope
AA 1095-1289
46
15
14
13
8
8
7
6
6
6
6
6
6
6
5
5
5
5
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
174
110
80
8
7
6
6
6
5
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
195
19
18
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
13
11
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
155
94
55
43
31
25
23
21
19
12
7
2
2
1
1
Supplier
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Immunogen Human nNOS aa. 1095-1289
Clone 16-nNOS-NOS Type I
Isotype IgG2a
Cross-Reactivity Rat (Rattus), Mouse (Murine), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name nNOS/NOS Type I (NOS1 Antibody Abstract)
Background Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+ levels and enhance calmodulin binding. Neuronal NOS (nNOS or bNOS) and endothelial NOS (ECNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and are regulated in a similar manner. However, both have been shown to be distinct gene products of about 155 kDa and 140 kDa, respectively, and the human forms show 52% amino acid identity. Neuronal NOS and induced macrophage NOS (iNOS) share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain. Neuronal NOS, a cytosolic protein present mainly in neural tissues, has been purified and characterized from rat cerebellum. The NO synthesized by this enzyme acts as a neurotransmitter. ECNOS has been cloned from human vascular endothelium as well as from bovine aortic endothelial cells (BAEC) and has a unique N-myristylation consensus sequence that may explain its membrane localization. This antibody is routinely tested by western blot analysis.
Synonyms: NOS Type I, Neuronal Nitric Oxide Synthase
Molecular Weight 155 kDa
Comment

Related Products: ABIN967389, ABIN968545

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Nitric Oxide Synthase 1, Neuronal (NOS1) (AA 1095-1289) antibody (ABIN967895) Western blot analysis of nNOS on a rat pituitary lysate. Lane 1: 1:2500, lane 2: 1:50...
Immunofluorescence (IF) image for anti-Nitric Oxide Synthase 1, Neuronal (NOS1) (AA 1095-1289) antibody (ABIN967895) Immunofluorescence staining on a rabbit brain section.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nitric Oxide Synthase 1, Neuronal (NOS1) (AA 1095-1289) antibody (ABIN967895) Immunohistochemical staining of a formalin-fixed paraffin-embedded rat brain tissue s...
Product cited in: Schuh, Uldrijan, Telkamp et al.: "The plasmamembrane calmodulin-dependent calcium pump: a major regulator of nitric oxide synthase I." in: The Journal of cell biology, Vol. 155, Issue 2, pp. 201-5, 2001 (PubMed).

Sasaki, Gonzalez-Zulueta, Huang et al.: "Dynamic regulation of neuronal NO synthase transcription by calcium influx through a CREB family transcription factor-dependent mechanism." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 15, pp. 8617-22, 2000 (PubMed).

Yu, Shao, Qian et al.: "Gene transfer of the neuronal NO synthase isoform to cirrhotic rat liver ameliorates portal hypertension." in: The Journal of clinical investigation, Vol. 105, Issue 6, pp. 741-8, 2000 (PubMed).

Bredt, Hwang, Glatt et al.: "Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase." in: Nature, Vol. 351, Issue 6329, pp. 714-8, 1991 (PubMed).

Nathan: "Nitric oxide as a secretory product of mammalian cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 6, Issue 12, pp. 3051-64, 1992 (PubMed).