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SMAD2 antibody (SMAD, Mothers Against DPP Homolog 2) (AA 27-172)

Details for Product anti-SMAD2 Antibody No. ABIN968105, Supplier: Log in to see
Antigen
  • 7120426M23Rik
  • fj43c06
  • hmad-2
  • hMAD-2
  • hsmad2
  • hSMAD2
  • JV18
  • jv18
  • jv18-1
  • JV18-1
  • Madh2
  • MADH2
  • madh2
  • madr2
  • MADR2
  • Madr2
  • mMad2
  • Smad-2
  • smad-2
  • wu:fj43c06
  • Xmad2
  • XSmad2
Alternatives
anti-Human SMAD2 antibody for Western Blotting
Epitope
AA 27-172
51
40
31
27
22
18
15
13
12
11
9
7
6
6
6
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
396
174
157
21
16
8
8
8
8
7
6
5
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
337
47
22
Clonality (Clone)
Monoclonal ()
Conjugate
This SMAD2 antibody is un-conjugated
14
12
10
8
8
8
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
330
185
80
80
48
35
28
23
17
9
6
6
5
3
2
Supplier
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Immunogen Human MAD2 aa. 27-172
Clone 48-MAD2
Isotype IgG2a
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name hsMAD2 (SMAD2 Antibody Abstract)
Background Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. These events are of utmost importance at the checkpoints, or transition points, of the cell cycle. MAD2 (Mitotic Arrest Deficient) is the human homolog of a yeast and Xenopus protein that is essential for spindle assembly during mitosis. The human hsMAD2 gene encodes a protein of 205 amino acids with a predicted molecular weight of 23.5 kDa. Binding of affinity purified polyclonal antibodies to the MAD2 protein prevents mitosis of HeLa cells. This indicates that, like its invertebrate relatives, MAD2 is necessary for mitosis. Furthermore, MAD2 is localized at the kinetochore of condensed chromosomes during mitosis and cells defective in the mitotic checkpoint have reduced levels of MAD2.
Synonyms: Mitotic Arrest Deficient-2
Molecular Weight 24 kDa
Pathways Cell Division Cycle
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-SMAD2 antibody (SMAD, Mothers Against DPP Homolog 2) (AA 27-172) (ABIN968105) Western blot analysis of MAD2 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TI...
Immunofluorescence (IF) image for anti-SMAD2 antibody (SMAD, Mothers Against DPP Homolog 2) (AA 27-172) (ABIN968105) Immunofluorescence staining of rabbit spleen.
Product cited in: Babu, Jeganathan, Baker, Wu, Kang-Decker, van Deursen: "Rae1 is an essential mitotic checkpoint regulator that cooperates with Bub3 to prevent chromosome missegregation." in: The Journal of cell biology, Vol. 160, Issue 3, pp. 341-53, 2003 (PubMed).

Iwanaga, Kasai, Kibler, Jeang: "Characterization of regions in hsMAD1 needed for binding hsMAD2. A polymorphic change in an hsMAD1 leucine zipper affects MAD1-MAD2 interaction and spindle checkpoint function." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 31005-13, 2002 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).

Chen, Waters, Salmon, Murray: "Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores." in: Science (New York, N.Y.), Vol. 274, Issue 5285, pp. 242-6, 1996 (PubMed).

Background publications Li, Benezra: "Identification of a human mitotic checkpoint gene: hsMAD2." in: Science (New York, N.Y.), Vol. 274, Issue 5285, pp. 246-8, 1996 (PubMed).