Aurora Kinase A (AURKA) (AA 8-116) antibody
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|4 references available|
|Price||Product not available in this region.|
|Cross-Reactivity||Human, Rat (Rattus)|
Cell division is a tightly regulated process that ensures the segregation of chromosomes into daughter cells. Essential to this regulation is the modification of cell cycle components by reversible phosphorylation. Ipl1 and aurora are two related kinases isolated from S.cerevisiae and Drosophila, respectively. Inactivation of these kinases results in abnormal chromosome segregation and disruption of the centrosome. A structurally and functionally similar kinase, IAK1 (Ipl1- and Aurora-related kinase 1), is a regulator of mammalian chromosome segregation. Although IAK1 may be present in the cytoplasm, it is detected on the centrosome following duplication and also associates with the spindle microtubules from metaphase through cell division. Expression of IAK1 is stringently regulated during the cell cycle. Both mRNA and protein are initially expressed in S-phase, are elevated during M-phase, and are undetectable following completion of mitosis. Increasing evidence suggests that IAK1 belongs to a novel subfamily of the ser/thr kinase superfamily. Although mutational analysis of IAK1 will directly determine its function, it appears to be a key player in the control of cell division.
Synonyms: Aurora-A Kinase
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||46 kDa|
Related Products: ABIN968537, ABIN967389
|Synonyms||AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, MGC34538, IAK, Ark1, Ayk1, Stk6, AIRK1, AU019385, AW539821, Aurora-A, 87A7-9/2, AurA, ck10, ck, l(3)87Ac, l(3)ck10, DmelCG3068, CG3068, MGC124935, aurka, AURKA, MGC134472, aik, ark1, aura, btak, stk6, stk7, stk15, aurora2, zgc:100912|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
|Western blot analysis of IAK1 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-IAK1 antibody. Immunofluorescence staining of human endothelial cells.|
Gopalan, Chan, Donovan: "A novel mammalian, mitotic spindle-associated kinase is related to yeast and fly chromosome segregation regulators." in: The Journal of cell biology, Vol. 138, Issue 3, pp. 643-56, 1997 (PubMed).
Katayama, Ota, Jisaki et al.: "Mitotic kinase expression and colorectal cancer progression." in: Journal of the National Cancer Institute, Vol. 91, Issue 13, pp. 1160-2, 1999 (PubMed).
Kiat, Hui, Gopalan: "Aurora-A kinase interacting protein (AIP), a novel negative regulator of human Aurora-A kinase." in: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 45558-65, 2002 (PubMed).
Sakai, Urano, Ookata et al.: "MBD3 and HDAC1, two components of the NuRD complex, are localized at Aurora-A-positive centrosomes in M phase." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48714-23, 2002 (PubMed).