Caspase 2 antibody (Caspase 2, Apoptosis-Related Cysteine Peptidase) (AA 225-401)

Details for Product anti-CASP2 Antibody No. ABIN968323, Supplier: Log in to see
Antigen
  • xCaspase-2
  • Caspase-2
  • ICH-1
  • Nedd2
  • CASP-2
  • ICH1
  • NEDD-2
  • NEDD2
  • PPP1R57
  • ICH-1L/1S
  • ICH1L1S
  • caspase-2-like
  • caspase-2
  • caspase 2
  • caspase 2, apoptosis-related cysteine peptidase
  • LOC100050611
  • xCaspase-2
  • CpipJ_CPIJ008254
  • Casp2
  • LOC100101590
  • CASP2
  • LOC100521118
Alternatives
anti-Human Caspase 2 antibody for BioImaging
Epitope
AA 225-401
27
21
14
9
7
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
209
90
69
10
8
2
2
2
2
2
1
1
1
1
1
1
Host
Mouse
156
50
13
Clonality (Clone)
Monoclonal ()
Conjugate
This Caspase 2 antibody is un-conjugated
6
6
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
179
98
47
35
17
13
13
12
12
4
4
4
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Human ICH-1L aa. 225-401
Clone 35-Caspase-2-ICH
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Caspase-2 (CASP2 Antibody Abstract)
Background Caspase-2/ICH-1 is related to the C. elegans cell death gene product CED-3 and its mammalian homologue interleukin-1beta-converting enzyme (ICE). Caspase-2 /ICH-1 was identified from a mouse cDNA library and originally termed NEDD-2. The NEDD-2 mRNA was found to be expressed during early mouse embryonic brain development and subsequently down-regulated in adult neuronal tissue. With the identification of the human NEDD-2 gene, the murine gene was renamed Ich-1 to symbolize Ice and ced-3 homology. Caspase-2/ICH-1 mRNA is alternatively spliced. The larger mRNA species encoding a product of 435 amino acids is known as Caspase-2 long, or ICH-1L. The smaller mRNA species encoding a protein of 312 amino acids is named Caspase-2 short, or ICH-1S. Overexpression of ICH-1L induces apoptosis, while over-expression of Ich-1S suppresses Rat-1 cell death induced by serum deprivation. Thus, it appears that Caspase-2/ICH-1 plays an important dual role in programmed cell death.
Synonyms: ICH-1L
Molecular Weight 48 kDa
Research Area Apoptosis/Necrosis, Cancer, Autophagy
Pathways Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Caspase 2 antibody (Caspase 2, Apoptosis-Related Cysteine Peptidase) (AA 225-401) (ABIN968323) Western blot analysis of Caspase-2 on Jurkat cell lysate. Lane 1: 1:1000, lane 2: 1:2...
Immunofluorescence (IF) image for anti-Caspase 2 antibody (Caspase 2, Apoptosis-Related Cysteine Peptidase) (AA 225-401) (ABIN968323) Immunofluorescent staining of FHS cells.
Product cited in: Guo, Srinivasula, Druilhe, Fernandes-Alnemri, Alnemri: "Caspase-2 induces apoptosis by releasing proapoptotic proteins from mitochondria." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13430-7, 2002 (PubMed).

Li, Chen, Sinogeeva, Gorospe, Wersto, Chrest, Barnes, Liu: "Arsenic trioxide promotes histone H3 phosphoacetylation at the chromatin of CASPASE-10 in acute promyelocytic leukemia cells." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49504-10, 2002 (PubMed).

Mancini, Machamer, Roy, Nicholson, Thornberry, Casciola-Rosen, Rosen: "Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis." in: The Journal of cell biology, Vol. 149, Issue 3, pp. 603-12, 2000 (PubMed).

Shibata, Hisahara, Hara, Yamawaki, Fukuuchi, Yuan, Okano, Miura: "Caspases determine the vulnerability of oligodendrocytes in the ischemic brain." in: The Journal of clinical investigation, Vol. 106, Issue 5, pp. 643-53, 2000 (PubMed).

Wang, Miura, Bergeron, Zhu, Yuan: "Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death." in: Cell, Vol. 78, Issue 5, pp. 739-50, 1994 (PubMed).