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ARNT antibody (Aryl Hydrocarbon Receptor Nuclear Translocator) (AA 461-574)

Details for Product anti-ARNT Antibody No. ABIN968355, Supplier: Log in to see
Antigen
  • ARNT
  • arnt1
  • Arnt1
  • bHLHe2
  • D3Ertd557e
  • Drnt
  • ESTM42
  • HIF-1-beta
  • HIF-1beta
  • hif-1beta
  • HIF1-beta
  • Hif1b
  • HIF1B
  • hif1b
  • HIF1BETA
  • hif1beta
  • mKIAA4051
  • tango
  • TANGO
  • W08714
  • xArnt
  • zgc:136664
Epitope
AA 461-574
13
10
10
9
9
6
6
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
152
60
54
24
22
22
15
3
2
1
Host
Mouse
76
62
20
Clonality (Clone)
Monoclonal ()
Conjugate
This ARNT antibody is un-conjugated
4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
130
52
50
39
31
25
15
12
10
7
4
3
3
2
1
1
Supplier
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Immunogen Human HIF-1beta/ARNT1 aa. 461-574
Clone 29-HIF
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name HIF-1beta (ARNT Antibody Abstract)
Background The Ah-receptor (AHR) is a ligand activated transcription factor that mediates the biological effects of agonists. AHR dimerizes with a structurally related protein known as ARNT (arylhydrocarbon-receptor nuclear transducer). This heterodimer binds enhancer elements and induces the expression of target genes, specifically those involved in the metabolism of xenobiotics. ARNT1 and ARNT2 are members of the basic-helix-loop-helix-PAS family of heterodimeric transcription factors, which also includes AHR, hypoxia-inducible factor-1alpha (HIF-1alpha), and the Drosophila single-minded protein (Sim). While ARNT2 expression is limited to brain and kidney, ARNT1 exhibits ubiquitous expression. A targeted disruption of the Arnt locus in the mouse yields embryonic stem cells that fail to activate genes that normally respond to low oxygen tension. Arnt -/- embryos do not survive and show defective angiogenesis of the yolk sac and branchial arches, stunted development, and wasting. Thus, in addition to its regulation of xenobiotic metabolism genes, ARNT is thought to induce developmental gene expression resulting in vascularization of the developing embryo.
Molecular Weight 95 kDa
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-ARNT antibody (Aryl Hydrocarbon Receptor Nuclear Translocator) (AA 461-574) (ABIN968355) Western blot analysis of HIF-1beta/ARNT1 on Jurkat cell lysate. Lane 1: 1:1000, lane ...
Immunofluorescence (IF) image for anti-ARNT antibody (Aryl Hydrocarbon Receptor Nuclear Translocator) (AA 461-574) (ABIN968355) Immunofluorescent staining of A431 cells.
Product cited in: Ambrosini, Nath, Sierra-Honigmann, Flores-Riveros: "Transcriptional activation of the human leptin gene in response to hypoxia. Involvement of hypoxia-inducible factor 1." in: The Journal of biological chemistry, Vol. 277, Issue 37, pp. 34601-9, 2002

Jiang, Jiang, Zheng, Lu, Hunter, Vogt: "Phosphatidylinositol 3-kinase signaling controls levels of hypoxia-inducible factor 1." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 12, Issue 7, pp. 363-9, 2001

Suzuki, Tomida, Tsuruo: "Dephosphorylated hypoxia-inducible factor 1alpha as a mediator of p53-dependent apoptosis during hypoxia." in: Oncogene, Vol. 20, Issue 41, pp. 5779-88, 2001

Maltepe, Schmidt, Baunoch, Bradfield, Simon: "Abnormal angiogenesis and responses to glucose and oxygen deprivation in mice lacking the protein ARNT." in: Nature, Vol. 386, Issue 6623, pp. 403-7, 1997

Drutel, Kathmann, Heron, Schwartz, Arrang: "Cloning and selective expression in brain and kidney of ARNT2 homologous to the Ah receptor nuclear translocator (ARNT)." in: Biochemical and biophysical research communications, Vol. 225, Issue 2, pp. 333-9, 1996