rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 906-1012) antibody

Antigen: rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1)

Synonyms: PRO0435, MGC43611, P160ROCK, MGC131603, LOC100229794, DROK, DRhk, ROCK, ROCK-1, ROCK1, ROK, ROKalpha/beta, Rhk, RhoK, DmelCG9774, CG9774

Binding Site: AA 906-1012

Clonality: Monoclonal (46)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968386

Additional Information

Alternative name: ROCK-I

Immunogen: Mouse ROCK-I

Cross-Reactivity: Human, Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 46

Description: ROCK-I is a Rho-associated serine/threonine kinase isozyme that mediates RhoA-induced assembly of focal adhesions and actin stress fibers. It contains an N-terminal kinase domain, a central 600 amino acid long coiled-coil region, a C-terminal pleckstrin homology region (PH) and a Cys-rich zinc finger motif. The ROCK-I kinase domain is approximately 90% identical to that of ROCK-II. ROCK-I binds GTP-bound Rho through a Rho-binding domain (RBD). As a result, the kinase activity of ROCK-I is moderately stimulated. The ROCK isozymes regulate cell contractility through phosphorylation of the myosin light chain. This effect results from either the inhibition of the myosin phosphatase or by direct phosphorylation of the myosin light chain, thus bypassing the myosin light chain kinase. In addition, ROCK-I activates the ubiquitously expressed Na-H exchanger (NHE1) via a number of mechanisms including RhoA. NHE1 may mediate ROCK-I-induced changes in the actin cytoskeleton. Therefore, ROCK-I plays an important role in the regulation of focal adhesion and stress fiber formation.
Synonyms: ROKbeta

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 160 kDa

Comments:

Related Products: ABIN968542, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of ROCK-I on mouse kidney lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-ROCK-I antibody.

Publications

Product: Ishizaki, Maekawa, Fujisawa et al.: "The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase." in: The EMBO journal, Vol. 15, Issue 8, pp. 1885-93, 1996 (PubMed).

Nakagawa, Fujisawa, Ishizaki et al.: "ROCK-I and ROCK-II, two isoforms of Rho-associated coiled-coil forming protein serine/threonine kinase in mice." in: FEBS letters, Vol. 392, Issue 2, pp. 189-93, 1996 (PubMed).

Sahai, Olson, Marshall: "Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility." in: The EMBO journal, Vol. 20, Issue 4, pp. 755-66, 2001 (PubMed).

Pawlak, Helfman: "Post-transcriptional down-regulation of ROCKI/Rho-kinase through an MEK-dependent pathway leads to cytoskeleton disruption in Ras-transformed fibroblasts." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 336-47, 2002 (PubMed).

Wang, Eto, Steers et al.: "RhoA-mediated Ca2+ sensitization in erectile function." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 30614-21, 2002 (PubMed).