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ROCK1 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 1) (AA 906-1012)

Details for Product anti-ROCK1 Antibody No. ABIN968386, Supplier: Login to see
Antigen
  • LOC100229794
  • CG9774
  • DROK
  • DRhk
  • DRok
  • Dmel\\CG9774
  • Drok
  • ROCK
  • ROCK-1
  • ROCK1
  • ROK
  • ROKalpha/beta
  • Rhk
  • RhoK
  • Rock
  • Rok
  • dROK
  • dRok
  • drok
  • 1110055K06Rik
  • Rock-I
  • P160Rock
  • ROCK-I
  • P160ROCK
  • p160ROCK
Alternatives
anti-Human ROCK1 antibody for Western Blotting
Epitope
AA 906-1012
25
24
13
12
11
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
118
72
69
3
3
2
2
2
1
Host
Mouse
106
17
Clonality (Clone)
Monoclonal ()
Conjugate
This ROCK1 antibody is un-conjugated
4
3
3
3
3
3
3
3
3
3
3
2
Application
Immunofluorescence (IF), Western Blotting (WB)
86
32
29
21
14
14
11
6
2
1
1
1
1
Supplier
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Immunogen Mouse ROCK-I aa. 906-1012
Clone 46-ROCK
Isotype IgG1
Cross-Reactivity Human, Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name ROCK-I (ROCK1 Antibody Abstract)
Background ROCK-I is a Rho-associated serine/threonine kinase isozyme that mediates RhoA-induced assembly of focal adhesions and actin stress fibers. It contains an N-terminal kinase domain, a central 600 amino acid long coiled-coil region, a C-terminal pleckstrin homology region (PH) and a Cys-rich zinc finger motif. The ROCK-I kinase domain is approximately 90% identical to that of ROCK-II. ROCK-I binds GTP-bound Rho through a Rho-binding domain (RBD). As a result, the kinase activity of ROCK-I is moderately stimulated. The ROCK isozymes regulate cell contractility through phosphorylation of the myosin light chain. This effect results from either the inhibition of the myosin phosphatase or by direct phosphorylation of the myosin light chain, thus bypassing the myosin light chain kinase. In addition, ROCK-I activates the ubiquitously expressed Na-H exchanger (NHE1) via a number of mechanisms including RhoA. NHE1 may mediate ROCK-I-induced changes in the actin cytoskeleton. Therefore, ROCK-I plays an important role in the regulation of focal adhesion and stress fiber formation.
Synonyms: ROKbeta
Molecular Weight 160 kDa
Pathways Microtubule Dynamics, WNT Signaling
Comment

Related Products: ABIN968542, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-ROCK1 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 1) (AA 906-1012) (ABIN968386) Western blot analysis of ROCK-I on mouse kidney lysate. Lane 1: 1:250, lane 2: 1:500,...
Product cited in: Wang, Eto, Steers et al.: "RhoA-mediated Ca2+ sensitization in erectile function." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 30614-21, 2002 (PubMed).

Pawlak, Helfman: "Post-transcriptional down-regulation of ROCKI/Rho-kinase through an MEK-dependent pathway leads to cytoskeleton disruption in Ras-transformed fibroblasts." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 336-47, 2002 (PubMed).

Sahai, Olson, Marshall: "Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility." in: The EMBO journal, Vol. 20, Issue 4, pp. 755-66, 2001 (PubMed).

Nakagawa, Fujisawa, Ishizaki et al.: "ROCK-I and ROCK-II, two isoforms of Rho-associated coiled-coil forming protein serine/threonine kinase in mice." in: FEBS letters, Vol. 392, Issue 2, pp. 189-93, 1996 (PubMed).

Ishizaki, Maekawa, Fujisawa et al.: "The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase." in: The EMBO journal, Vol. 15, Issue 8, pp. 1885-93, 1996 (PubMed).