ROCK1 antibody (pSer456, pThr455)

Catalog No. ABIN707771

Product Details anti-ROCK1 Antibody

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Binding Specificity: pSer456, pThr455

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROCK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ROCK1 around the phosphorylation site of Thr455/Ser456

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ROCK1 antibody (ABIN707771)

Lin, Chang, Chen, Shih: "RhoA/ROCK1 regulates Avian Reovirus S1133-induced switch from autophagy to apoptosis." in: BMC veterinary research, Vol. 11, pp. 103, (2015) (PubMed).

Choi, Park, Kim, Park, Lee, Choi: "GM-CSF reduces expression of chondroitin sulfate proteoglycan (CSPG) core proteins in TGF-β-treated primary astrocytes." in: BMB reports, Vol. 47, Issue 12, pp. 679-84, (2015) (PubMed).

Wang, Zhang, Wang, Zhang, Mao, Zhuang, Tang, Luo, Zhou, Zhang: "Vascular endothelial growth factor stimulates endothelial differentiation from mesenchymal stem cells via Rho/myocardin-related transcription factor--a signaling pathway." in: The international journal of biochemistry & cell biology, Vol. 45, Issue 7, pp. 1447-56, (2013) (PubMed).

Target Details for ROCK1

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Alternative Name: ROCK1 + (ROCK1 Products)

Synonyms: LOC100229794 antibody, CG9774 antibody, DROK antibody, DRhk antibody, DRok antibody, Dmel\\CG9774 antibody, Drok antibody, ROCK antibody, ROCK-1 antibody, ROCK1 antibody, ROK antibody, ROKalpha/beta antibody, Rhk antibody, RhoK antibody, Rock antibody, Rok antibody, dROK antibody, dRok antibody, drok antibody, 1110055K06Rik antibody, Rock-I antibody, P160Rock antibody, ROCK-I antibody, P160ROCK antibody, p160ROCK antibody, Rho associated coiled-coil containing protein kinase 1 antibody, Rho kinase antibody, Rho-associated coiled-coil containing protein kinase 1 antibody, Rho-associated, coiled-coil containing protein kinase 1 antibody, ROCK1 antibody, Rok antibody, Rock1 antibody, rock1 antibody

Background:

Synonyms: ROCK-I, P16ROCK, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, Rho-associated, coiled-coil-containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, p16 ROCK-1, ROCK1

Background: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.

Gene ID: 6093

UniProt: Q13464

Pathways: Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling