ROCK1 antibody (rho-Associated, Coiled-Coil Containing Protein Kinase 1)

Details for Product anti-ROCK1 Antibody No. ABIN969563
Target
Reactivity
Human
95
67
62
7
4
4
4
2
2
1
1
1
Host
Mouse
88
8
Clonality (Clone)
Monoclonal ()
Conjugate
This ROCK1 antibody is un-conjugated
5
4
4
4
4
4
4
3
2
2
2
2
2
2
Application
ELISA, Western Blotting (WB)
60
26
23
20
15
13
11
9
7
3
2
2
1
1
1
Options
Immunogen Purified recombinant fragment of human ROCK1 expressed in E. Coli.
Clone 1H4
Isotype IgG1
Purification Purified
Target
Alternative Name ROCK1 (ROCK1 Antibody Abstract)
Background This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity.
Molecular Weight 158 kDa
Gene ID 6093
HGNC 6093
Pathways Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage
Images
Image no. 1 for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) antibody (ABIN969563) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Image no. 2 for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) antibody (ABIN969563) Figure 2: Western blot analysis using ROCK1 mAb against human ROCK1 (AA: 403-610) rec...
Image no. 3 for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) antibody (ABIN969563) Figure 3: Western blot analysis using ROCK1 mAb against HEK293 (1) and ROCK1 (AA: 403...
Product cited in: Kroll, Epting, Kern, Dietz, Feng, Hammes, Wieland, Augustin: "Inhibition of Rho-dependent kinases ROCK I/II activates VEGF-driven retinal neovascularization and sprouting angiogenesis." in: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 3, pp. H893-9, 2009 (PubMed).

Narumiya, Tanji, Ishizaki: "Rho signaling, ROCK and mDia1, in transformation, metastasis and invasion." in: Cancer metastasis reviews, Vol. 28, Issue 1-2, pp. 65-76, 2009 (PubMed).