ROCK1 antibody (AA 401-510)

Catalog No. ABIN562671

Cited in 2+ publications. The Mouse Monoclonal anti-ROCK1 antibody (Clone 2E2) (ABIN562671) specifically detects ROCK1 in WB, ELISA and IF. The antibody is reactive with Human samples.

Quick Overview for ROCK1 antibody (AA 401-510) (ABIN562671)

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ROCK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 2E2

Product Details anti-ROCK1 Antibody

Binding Specificity: AA 401-510

Purpose: Mouse monoclonal antibody raised against a partial recombinant ROCK1.

Sequence: SNRRYLSSAN PNDNRTSSNA DKSLQESLQK TIYKLEEQLH NEMQLKDEME QKCRTSNIKL DKIMKELDEE GNQRRNLEST VSQIEKEKML LQHRINEYQR KAEQENEKRR

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: ROCK1 (NP_005397, 401 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-ROCK1 antibody (ABIN562671)

Nishimura, Bereczky, Yoshioka, Taniguchi, Itoh: "A novel role of Rho-kinase in the regulation of ligand-induced phosphorylated EGFR endocytosis via the early/late endocytic pathway in human fibrosarcoma cells." in: Journal of molecular histology, Vol. 42, Issue 5, pp. 427-42, (2011) (PubMed).

Gong, Fang, Peng, Liu, Du: "Integration of virtual screening with high-throughput screening for the identification of novel Rho-kinase I inhibitors." in: Journal of biotechnology, Vol. 145, Issue 3, pp. 295-303, (2010) (PubMed).

Target Details for ROCK1

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Alternative Name: ROCK1

Background: Full Gene Name: Rho-associated, coiled-coil containing protein kinase 1
Synonyms: MGC131603,MGC43611,P160ROCK,PRO0435

Gene ID: 6093

NCBI Accession: NM_005406

Pathways: Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling