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Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1) (AA 73-186) antibody

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Synonyms TGFB1I1, hic5, ara55, hic-5, tsc-5, ARA55, HIC-5, HIC5, TSC-5, Hic5, Ara55, Hic-5, ara-55
AA 73-186
(5), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(35), (17), (14), (2)
(34), (7), (1)
Clonality (Clone)
Monoclonal ()
(2), (2), (1)
Immunofluorescence (IF), Western Blotting (WB)
(31), (22), (8), (7), (5), (5), (2), (2), (1), (1), (1)
Pubmed 4 references available
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Quantity 50 μg
Shipping to United States ( )
Immunogen Mouse Hic-5 aa. 73-186
Clone 34-Hic
Isotype IgG1
Cross-Reactivity Rat (Rattus), Human, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Hic-5 (TGFB1I1 Antibody Abstract)
Background Focal adhesions are cell structures terminal to the actin-stress fiber bundles. They attach cultured cells to their substratum or extracellular matrix. These adhesions occur through the integrin receptor molecules which link cytoskeletal proteins and extracellular matrix. The intracellular adhesion is composed of a number of proteins such as paxillin, VASP, vinculin, and the focal-adhesion kinases FAK and PYK2/CAKbeta. Hic-5, also described as hydrogen peroxide inducible-mRNA, is a focal adhesion protein that binds to FAK and PYK2. It is ubiquitously expressed with the highest levels found in lung, spleen, and heart. Induction of Hic-5 is accomplished by hydrogen peroxide or TGFbeta1 and is repressed in K-ras transformed cells. Like paxillin, Hic-5 is tyrosine phosphorylated in Src-transformed cells and is highly similar to paxillin in its primary structure. Both proteins contain LIM domains and LD motifs. In addition, Hic-5 localizes to focal adhesions and co-immunoprecipitates with PYK2/CAKbeta in vivo. Thus, Hic-5 may be a substrate for CAKbeta and play a role in signal transduction during proliferation, cell motility, and adhesion.
Molecular Weight 50 kDa

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1) (AA 73-186) antibody (ABIN968400) Western blot analysis of Hic-5 on a rat lung lysate. Lane 1: 1:250, lane 2: 1:500, la...
Immunofluorescence (IF) image for anti-Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1) (AA 73-186) antibody (ABIN968400) Immunofluorescence staining of human fibroblasts.
Product cited in: Wang, Yang, Guo et al.: "Suppression of androgen receptor transactivation by Pyk2 via interaction and phosphorylation of the ARA55 coregulator." in: The Journal of biological chemistry, Vol. 277, Issue 18, pp. 15426-31, 2002 (PubMed).

Jia, Ransom, Shibanuma et al.: "Identification and characterization of hic-5/ARA55 as an hsp27 binding protein." in: The Journal of biological chemistry, Vol. 276, Issue 43, pp. 39911-8, 2001 (PubMed).

Shibanuma, Mashimo, Kuroki et al.: "Characterization of the TGF beta 1-inducible hic-5 gene that encodes a putative novel zinc finger protein and its possible involvement in cellular senescence." in: The Journal of biological chemistry, Vol. 269, Issue 43, pp. 26767-74, 1994 (PubMed).

Background publications Matsuya, Sasaki, Aoto et al.: "Cell adhesion kinase beta forms a complex with a new member, Hic-5, of proteins localized at focal adhesions." in: The Journal of biological chemistry, Vol. 273, Issue 2, pp. 1003-14, 1998 (PubMed).

Catalog No. ABIN968400
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