ITCH antibody (AA 114-220)

Catalog No. ABIN968414

The Mouse Monoclonal anti-ITCH antibody has been validated for WB and IF. It is suitable to detect ITCH in samples from Human, Mouse, Rat and Rabbit. There is 1 publication available.

Quick Overview for ITCH antibody (AA 114-220) (ABIN968414)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITCH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 32-Itch

Product Details anti-ITCH Antibody

Binding Specificity: AA 114-220

Cross-Reactivity: Rat (Rattus), Human, Rabbit

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Itch aa. 114-220

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968548, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-ITCH antibody (ABIN968414)

Perry, Hustad, Swing, OSullivan, Jenkins, Copeland: "The itchy locus encodes a novel ubiquitin protein ligase that is disrupted in a18H mice." in: Nature genetics, Vol. 18, Issue 2, pp. 143-6, (1998) (PubMed).

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: Itch

Background: Maintenance of cellular function requires timely and selective degradation of key regulatory proteins. For example, progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins via the ubiquitin pathway. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an epsilon-NH2-Lys in a target protein. Ubiquitin-conjugated proteins are recognized and degraded by the 26S proteasome. Ubiquitination requires ubiquitin-activating enzyme E1, ubiquitin-conjugating enzymes E2, and ubiquitin ligases E3. The direction of ubiquitin transfer is from E1 to E2 and from E2 to E3. Itch, a novel E3 ubiquitin ligase, is absent in the Non-agouti-lethal 18H mice. These mice develop immunological, inflammatory, epithelial, and hematopoietic diseases. Itch contains four WW protein interaction domains, which bind to proline-rich sequences in a fashion similar to SH3 domains. In addition, Itch contains a C-terminal Hect domain, which is conserved in the E3 family of ubiquitin ligases. Thus, Itch is important in the ubiquitin-dependent protein degradation occurring in normal hematopoiesis and inflammation.

Molecular Weight: 113 kDa

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events