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RPS6KB1 antibody (AA 2-121)

RPS6KB1 Reactivity: Human, Mouse, Rat WB, IF Host: Mouse Monoclonal 16-p70[s6k] unconjugated
Catalog No. ABIN968441
  • Target See all RPS6KB1 Antibodies
    RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))
    Binding Specificity
    • 29
    • 28
    • 21
    • 19
    • 18
    • 16
    • 16
    • 16
    • 15
    • 15
    • 15
    • 15
    • 14
    • 13
    • 12
    • 11
    • 10
    • 8
    • 8
    • 8
    • 8
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-121
    Reactivity
    • 354
    • 260
    • 257
    • 57
    • 24
    • 23
    • 20
    • 19
    • 11
    • 9
    • 9
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    Human, Mouse, Rat
    Host
    • 334
    • 33
    • 4
    • 2
    Mouse
    Clonality
    • 336
    • 37
    Monoclonal
    Conjugate
    • 171
    • 24
    • 17
    • 15
    • 15
    • 15
    • 15
    • 15
    • 15
    • 15
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 6
    • 1
    • 1
    • 1
    This RPS6KB1 antibody is un-conjugated
    Application
    • 294
    • 147
    • 104
    • 94
    • 91
    • 77
    • 66
    • 32
    • 24
    • 24
    • 20
    • 8
    • 7
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Cross-Reactivity
    Mouse (Murine), Human
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Rat p70 [S6K] aa. 2-121
    Clone
    16-p70[s6k]
    Isotype
    IgG1
    Top Product
    Discover our top product RPS6KB1 Primary Antibody
  • Comment

    Related Products: ABIN968548, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Brennan, Babbage, Thomas, Cantrell: "p70(s6k) integrates phosphatidylinositol 3-kinase and rapamycin-regulated signals for E2F regulation in T lymphocytes." in: Molecular and cellular biology, Vol. 19, Issue 7, pp. 4729-38, (1999) (PubMed).

    Johanson, Naccache, Crouch: "A p85 subunit-independent p110alpha PI 3-kinase colocalizes with p70 S6 kinase on actin stress fibers and regulates thrombin-stimulated stress fiber formation in swiss 3T3 cells." in: Experimental cell research, Vol. 248, Issue 1, pp. 223-33, (1999) (PubMed).

    Romanelli, Martin, Toker, Blenis: "p70 S6 kinase is regulated by protein kinase Czeta and participates in a phosphoinositide 3-kinase-regulated signalling complex." in: Molecular and cellular biology, Vol. 19, Issue 4, pp. 2921-8, (1999) (PubMed).

    Ming, Burgering, Wennström, Claesson-Welsh, Heldin, Bos, Kozma, Thomas: "Activation of p70/p85 S6 kinase by a pathway independent of p21ras." in: Nature, Vol. 371, Issue 6496, pp. 426-9, (1994) (PubMed).

  • Target
    RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))
    Alternative Name
    p70 s6k (RPS6KB1 Products)
    Synonyms
    PS6K antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, STK14A antibody, p70 S6KA antibody, p70(S6K)-alpha antibody, p70-S6K antibody, p70-alpha antibody, 10539 antibody, 7084 antibody, CG10539 antibody, DS6K antibody, Dmel\\CG10539 antibody, Dp70S6k antibody, Dp70[s6k] antibody, Dp70s6k antibody, S6k antibody, dS6K antibody, dS6k antibody, dp70[S6k] antibody, dp70s6k antibody, dps6k antibody, ds6k antibody, fs(3)07084 antibody, l(3)07084 antibody, p70 S6K antibody, p70/S6K antibody, p70S6K antibody, p70[S6 kinase] antibody, p70[S6K] antibody, p70[S6k] antibody, p70[S6kinase] antibody, p70s6K antibody, s6k antibody, s6k11 antibody, 2610318I15Rik antibody, 4732464A07Rik antibody, 70kDa antibody, AA959758 antibody, AI256796 antibody, AI314060 antibody, p70/85s6k antibody, p70s6k antibody, p70s6k-A antibody, p70-s6k antibody, ps6k antibody, rps6kb1 antibody, rps6kb1-A antibody, s6K1 antibody, stk14a antibody, fc51h01 antibody, wu:fc51h01 antibody, zgc:55713 antibody, ribosomal protein S6 kinase B1 antibody, Ribosomal protein S6 kinase antibody, ribosomal protein S6 kinase, polypeptide 1 antibody, ribosomal protein S6 kinase B1 L homeolog antibody, ribosomal protein S6 kinase B1 S homeolog antibody, ribosomal protein S6 kinase b, polypeptide 1b antibody, RPS6KB1 antibody, S6k antibody, Rps6kb1 antibody, rps6kb1.L antibody, rps6kb1.S antibody, rps6kb1b antibody
    Background
    Increased protein synthesis, an obligatory step in cell proliferation, is regulated by the phosphorylation of a number of translational components, such as ribosomal protein S6. p70 S6 Kinase (p70 [S6K]), a cytosolic kinase that is primarily responsible for S6 phosphorylation, belongs to a family of at least six S6 kinases. p70 [S6K] and p85 [S6K] are two isoforms of the same kinase. Although they are encoded by the same gene, p80 [S6K] contains a 23 amino acid N-terminal extension that serves as a nuclear localization sequence. These kinases are located in a p21 ras-independent signaling pathway and are regulated by Ser/Thr phosphorylation in response to mitogenic stimulation. Activation of p70 [S6K] requires sequential phosphorylations within the autoinhibitory domain and at Thr 389. p70 [S6K] associates with PDK-1 and PKCzeta to form a possible PI3-K signaling complex. The immunosuppressive agent rapamycin forms an intracellular complex with FKBP12 and FRAP (RAFT1) that inhibits phosphorylation of p70 [S6K] at Thr 389 and, in turn, prevents enzyme activation. Thus, p70 [S6K] is an important regulator of cell proliferation and a potential target of agents that modify the immune and proliferative responses. p70 [S6K] has been reported to be observable migrating within a range of 60-70 kD by western blotting.
    Synonyms: p70 S6 Kinase
    Molecular Weight
    60-70 kDa
    Pathways
    PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect
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