Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pSer1177) antibody

Details for Product No. ABIN968845
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Antigen
Synonyms NOS3, ECNOS, eNOS, NOS, EC-NOS, NOSIII, cNOS, 2310065A03Rik, Nos-3, ecNOS, eNos, ENOS
Epitope
pSer1177
(32), (27), (25), (18), (17), (16), (15), (15), (15), (13), (11), (10), (8), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(317), (203), (199), (80), (69), (65), (37), (3), (2), (1), (1), (1)
Host
Mouse
(336), (22)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(17), (12), (12), (8), (8), (8), (8), (8), (8), (8), (8), (3), (3), (3)
Application
Western Blotting (WB), Flow Cytometry (FACS)
(224), (138), (80), (70), (48), (31), (29), (12), (12), (10), (9), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 50 µg
Options
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Catalog No. ABIN968845
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Immunogen Human eNOS (pS1177)
Clone Hs-3
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name eNOS
Background Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits signals involved in vasorelaxation, neurotransmission, and cytotoxity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+ levels and enhance calmodulin binding. Neuronal NOS (nNOS) and endothelial NOS (eNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin. eNOS has a unique N-myristylation consensus sequence that may explain its membrane localization. Various protein kinases have been implicated in regulation of eNOS activity, including AMPK, PKA, PKB/Akt, PKC, and CaM Kinase II. During VEGF stimulation, eNOS is transiently phosphorylated at Ser-1177 by PKB/akt and dephosphorylated at Thr-495. At later time points, VEGF stimulation leads to an increase in Thr-495 phosphorylation mediated by PKC and a decrease in Ser-1177 phosphorylation. In addition, Ser-633 and Ser-1177 are phosphorylated by PKA and PKG in vitro. Thus, eNOS activity may be regulated through complex phosphorylated events mediated by multiple kinases at various phosphorylation sites. Human endothelial cells are routinely tested as a positive control for eNOS (pS1177) mAb. 100% homology is detected for immunogen sequence in human, mouse, rat, dog and bovine. Cross-reactivity with other species is expected but not confirmed.
Molecular Weight 140 kDa
Research Area Cancer, Enzymes, Metabolism, Inflammation
Comment

Related Products: ABIN968536, ABIN967389, ABIN967886

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pSer1177) antibody Human Endothelial lysate was either left untreated (left column) or treated (right column) with 150 U/ml) of lambda phosphatase for 1 hour at 37°C. The top panel was probed with eNOS (ABIN967886). The bottom panel was probed with eNOS (pS1177) (ABIN968845).
Product cited in: Dimmeler, Fleming, Fisslthaler et al.: "Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation." in: Nature, Vol. 399, Issue 6736, pp. 601-5, 1999 (PubMed).

Gallis, Corthals, Goodlett et al.: "Identification of flow-dependent endothelial nitric-oxide synthase phosphorylation sites by mass spectrometry and regulation of phosphorylation and nitric oxide production by the phosphatidylinositol 3-kinase inhibitor LY294002." in: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 30101-8, 1999 (PubMed).

Michell, Chen Zp, Tiganis et al.: "Coordinated control of endothelial nitric-oxide synthase phosphorylation by protein kinase C and the cAMP-dependent protein kinase." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17625-8, 2001 (PubMed).

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