ENOS antibody (pThr495)
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- Target See all ENOS (NOS3) Antibodies
- ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
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Binding Specificity
- pThr495
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Reactivity
- Human, Rat, Mouse, Dog
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This ENOS antibody is un-conjugated
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Application
- Western Blotting (WB)
- Cross-Reactivity
- Mouse (Murine), Rat (Rattus), Dog (Canine)
- Characteristics
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Human Phosphorylated eNOS Peptide
- Clone
- 31-eNOS
- Isotype
- IgG1
- Top Product
- Discover our top product NOS3 Primary Antibody
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- Comment
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Related Products: ABIN968536, ABIN967389
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store undiluted at -20°C.
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Coordinated control of endothelial nitric-oxide synthase phosphorylation by protein kinase C and the cAMP-dependent protein kinase." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17625-8, (2001) (PubMed).
: "
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Coordinated control of endothelial nitric-oxide synthase phosphorylation by protein kinase C and the cAMP-dependent protein kinase." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17625-8, (2001) (PubMed).
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- Target
- ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
- Alternative Name
- eNOS (NOS3 Products)
- Synonyms
- ECNOS antibody, eNOS antibody, 2310065A03Rik antibody, Nos-3 antibody, ecNOS antibody, EC-NOS antibody, NOSIII antibody, cNOS antibody, NOS antibody, NOS3 antibody, eNos antibody, ENOS antibody, nitric oxide synthase 3 antibody, nitric oxide synthase 3, endothelial cell antibody, NOS3 antibody, Nos3 antibody, nos3 antibody
- Background
- Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+ levels and enhance calmodulin binding. Neuronal NOS (nNOS) and endothelial NOS (eNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin. eNOS has a unique N-myristylation consensus sequence that may explain its membrane localization. Various protein kinases have been implicated in regulation of eNOS activity, including AMPK, PKA, PKB/Akt, PKC, and CaM Kinase II. During VEGF stimulation, eNOS is transiently phosphorylated at Ser-1177 by PKB/akt and dephosphorylated at Thr-495. At later time points, VEGF stimulation leads to an increase in Thr-495 phosphorylation mediated by PKC and a decrease in Ser-1177 phosphorylation. In addition, Ser-495, Ser-633, and Ser-1177 are phosphorylated by PKA and PKG in vitro. Thus, eNOS activity may be regulated through complex phosphorylation events mediated by multiple kinases at various phosphorylation sites.
- Molecular Weight
- 140 kDa
- Pathways
- ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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