RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1)

Details for Product anti-RAF1 Antibody No. ABIN969559, Supplier: Log in to see
Antigen
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Alternatives
anti-Human RAF1 antibody for Enzyme Immunoassay
Reactivity
Human
834
412
387
25
23
16
16
13
8
8
8
5
4
4
2
2
1
1
1
Host
Mouse
780
62
12
6
2
Clonality (Clone)
Monoclonal ()
Conjugate
This RAF1 antibody is un-conjugated
45
45
44
30
29
29
14
14
12
12
12
12
12
12
12
12
12
2
2
2
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
512
364
220
172
156
104
52
48
20
18
18
7
2
2
2
1
Options
Supplier
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Supplier Product No.
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Immunogen Purified recombinant fragment of human RAF1 expressed in E. Coli.
Clone 4G4
Isotype IgG1
Purification Purified
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Gene ID 5894
HGNC 5894
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage
Supplier Images
ELISA image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 2: Flow cytometric analysis of Hela cells using RAF1 mouse mAb (green) and neg...
Immunohistochemistry (IHC) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues usin...
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 4: Western blot analysis using RAF1 mouse mAb against Hela (1), A431 (2), Cos7...
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969559) Figure 5: Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recom...
Product cited in: Hofmann, Heinrich, Radziwill, Radziwil, Moelling: "A short hairpin DNA analogous to miR-125b inhibits C-Raf expression, proliferation, and survival of breast cancer cells." in: Molecular cancer research : MCR, Vol. 7, Issue 10, pp. 1635-44, 2009 (PubMed).

Talmud, Drenos, Shah, Shah, Palmen, Verzilli, Gaunt, Pallas, Lovering, Li, Casas, Sofat, Kumari, Rodriguez, Johnson, Newhouse, Dominiczak, Samani, Caulfield, Sever, Stanton, Shields, Padmanabhan et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. ..." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

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