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anti-Human BID antibody for Immunoprecipitation

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist-like
  • BH3-interacting domain death agonist-like
  • BID
  • bida
  • bid
  • Bid
  • LOC100355991
  • LOC100730546
Epitope
Center
56
37
36
19
17
15
14
11
10
8
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
318
171
73
8
5
3
1
Host
Rabbit
291
94
20
9
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
7
7
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
342
185
125
83
52
41
32
20
13
11
11
6
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN2855009
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN222962 IP IHC WB Rabbit IgG Log in to see Polyclonal 6
1 ABIN127033 IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN4284618 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 1
1 ABIN957168 IHC (fro) IHC (p) IP WB Rabbit AA 28-50 Log in to see Polyclonal
1 ABIN4284613 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 1
1 ABIN4284615 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 3
1 ABIN1996891 ELISA ELISA (Detection) IP WB Mouse IgG2a AA 1-195 Log in to see 3C4C2
1 ABIN371640 EIA IP WB Mouse IgG2b Log in to see SB50a 10
1 ABIN957167 IHC (fro) IHC (p) IP WB Rabbit AA 1-55 Log in to see Polyclonal
1 ABIN1996899 IP ELISA WB Rabbit IgG AA 1-195 Log in to see 002
1 ABIN957166 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN1996905 IP ELISA WB Rabbit IgG AA 1-195 Log in to see Polyclonal
1 ABIN331205 IP ELISA WB Mouse IgG2a, kappa Log in to see
1 ABIN331391 IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN600516 FACS IP IHC ELISA WB Mouse IgG AA 83-103 Log in to see
1 ABIN1077861 ICC IHC IP WB Rabbit IgG AA 1-195 Log in to see Polyclonal
1 ABIN230728 IP ELISA WB Rabbit IgG Cleavage Site Log in to see Polyclonal
1 ABIN241684 IP IHC WB Rabbit Log in to see Polyclonal
1 ABIN408798 IP ELISA WB Rabbit AA 83-103 Log in to see Polyclonal
1 ABIN1584098 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal

Top referenced anti-BID antibody for Immunoprecipitation

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General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Epitope Center
(56), (37), (36), (19), (17), (15), (14), (11), (10), (8), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(318), (171), (73), (8), (5), (3), (1)
Host Rabbit
(291), (94), (20), (9)
Clonality
Polyclonal
Conjugate This BID antibody is un-conjugated
(21), (19), (14), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(342), (185), (125), (83), (52), (41), (32), (20), (13), (11), (11), (6), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-BID Antibody

Target Details BID Application Details Handling Images
Characteristics Rabbit polyclonal antibody to Bid (BH3 interacting domain death agonist)
Bid antibody [N1C3]
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human Bid. The exact sequence is proprietary.
Isotype IgG

Target Details BID

Product Details anti-BID Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bid (BID Antibody Abstract)
Background This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.

Cellular Localization: Cytoplasm , Mitochondrion membrane , BH3-interacting domain death agonist p15: Mitochondrion membrane , BH3-interacting domain death agonist p13: Mitochondrion membrane , Isoform 1: Cytoplasm , Isoform 3: Cytoplasm , Isoform 2: Mitochondrion membrane
Molecular Weight 22 kDa
Gene ID 637
Research Area Cancer, Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BID Antibody Target Details BID Handling Images back to top
Application Notes Suggested dilution Reference Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: A431 , H1299 , HeLa , HepG2

Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details Images back to top
Format Liquid
Concentration 0.8 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling back to top
Supplier Images
Immunoprecipitation (IP) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (Center) (ABIN2855009) IP Image Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 μ...
Western Blotting (WB) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (Center) (ABIN2855009) WB Image Sample (30 ug of whole cell lysate) A: A431 , 12% SDS PAGE antibody diluted ...
Immunoprecipitation (IP) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (Center) (ABIN2855009) IP Image Bid antibody [N1C3] immunoprecipitates BID protein in IP experiments. IP sam...