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anti-Mouse (Murine) BID antibody for Immunocytochemistry

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • DKFZp469E066
  • FP497
  • fp497
  • si:ch211-238n5.6
  • xbid
Epitope
AA 1-195
49
43
34
17
16
14
13
11
9
8
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
304
136
61
4
2
2
1
Host
Rabbit
276
90
21
10
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
7
7
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
310
186
118
70
40
34
30
19
13
12
12
3
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN3042321
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009965416 ABIN1077862 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 1-195 Log in to see Polyclonal
0.0009965416 ABIN4284622 ICC IF WB Mouse IgG Log in to see 23F7
0.0009965416 ABIN3030163 ICC IHC (p) WB Rabbit IgG AA 1-195 Log in to see Polyclonal

Similar anti-BID Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 13 Antibodies 48 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 4 Antibodies
Immunofluorescence (IF) 3 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 21 Antibodies 43 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 32 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 6 Antibodies

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Epitope AA 1-195
(49), (43), (34), (17), (16), (14), (13), (11), (9), (8), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(304), (136), (61), (4), (2), (2), (1)
Host Rabbit
(276), (90), (21), (10)
Clonality
Polyclonal
Conjugate This BID antibody is un-conjugated
(21), (19), (14), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(310), (186), (118), (70), (40), (34), (30), (19), (13), (12), (12), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-BID Antibody

Target Details BID Application Details Handling Images
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for BH3-interacting domain death agonist(BID) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: BH3 interacting domain death agonist
Protein Name: BH3-interacting domain death agonist
Purification Immunogen affinity purified.
Immunogen E.coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively.
Isotype IgG

Target Details BID

Product Details anti-BID Antibody Application Details Handling Images back to top
Antigen
Alternative Name BID (BID Antibody Abstract)
Background BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.
Gene ID 637
UniProt P55957
Research Area Cancer
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BID Antibody Target Details BID Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Bid is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 1-195) (ABIN3042321) Anti-Bid Picoband antibody, IHC(P): Human Mammary Cancer Tissue
Immunohistochemistry (IHC) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 1-195) (ABIN3042321) Anti-Bid Picoband antibody, ICC: A549 Cell
Western Blotting (WB) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 1-195) (ABIN3042321) anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 1-195) (ABIN3042321) Anti-Bid Picoband antibody, IHC(P): Mouse Spleen Tissue
Immunohistochemistry (IHC) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 1-195) (ABIN3042321) Anti-Bid Picoband antibody, IHC(P): Rat Intestine Tissue