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anti-Rat (Rattus) CAPNL1 antibody for Immunohistochemistry (Frozen Sections)

Recommended CAPNL1 Antibody (supplied by: Log in to see )

Antigen
Calpain 1, Large Subunit (CAPNL1) Antibodies
  • CANP
  • CANP1
  • CANPL1
  • Capa-1
  • Capa1
  • mu-calpin
  • muCANP
  • muCL
  • si:ch211-203k12.4
  • si:dkeyp-101g1.3
Epitope
AA 312-326, Middle Region
5
4
3
3
3
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
46
15
14
1
1
Host
Rabbit
30
18
4
Clonality
Polyclonal
Conjugate
This CAPNL1 antibody is un-conjugated
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
46
30
8
5
4
3
3
2
1
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Catalog No. ABIN3044292
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088051456 ABIN1858232 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 387-694 Log in to see Polyclonal

Similar anti-CAPNL1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 7 Antibodies 7 Antibodies 28 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 2 Antibodies 2 Antibodies
Immunofluorescence (IF) 1 Antibodies 1 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 6 Antibodies 6 Antibodies 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 3 Antibodies
Western Blotting (WB) 13 Antibodies 14 Antibodies

General

Antigen Calpain 1, Large Subunit (CAPNL1) Antibodies
Epitope AA 312-326, Middle Region
(5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(46), (15), (14), (1), (1)
Host Rabbit
(30), (18), (4)
Clonality
Polyclonal
Conjugate This CAPNL1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(46), (30), (8), (5), (4), (3), (3), (2), (1)
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Product Details anti-CAPNL1 Antibody

Target Details CAPNL1 Application Details Handling Images
Sequence EWNNVDPYER DQLRV
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Calpain-1 catalytic subunit(CAPN1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: calpain 1,(mu/I) large subunit
Protein Name: Calpain-1 catalytic subunit
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1(312-326aa EWNNVDPYERDQLRV), different from the mouse sequence by two amino acids.
Isotype IgG

Target Details CAPNL1

Product Details anti-CAPNL1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CAPN1 (CAPNL1 Antibody Abstract)
Background Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I(mu-calpain) and calpain II(m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L(large, catalytic, 80 kD) and S(small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1(CAPN1) and L2. By quantitative RT-PCR, Ueyama et al.(1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy(e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis(ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al.(1989, 1990) assign CANPL1 to chromosome 11.
UniProt P07384
Pathways Apoptosis

Application Details

Product Details anti-CAPNL1 Antibody Target Details CAPNL1 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-CAPNL1 Antibody Target Details CAPNL1 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-CAPNL1 Antibody Target Details CAPNL1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CAPNL1 antibody (Calpain 1, Large Subunit) (AA 312-326) (ABIN3044292) Anti-Calpain 1 antibody, IHC(P) IHC(P): Rat Lung Tissue
Immunohistochemistry (IHC) image for anti-CAPNL1 antibody (Calpain 1, Large Subunit) (AA 312-326) (ABIN3044292) Anti-Calpain 1 antibody, ICC ICC: HELA Cell
Western Blotting (WB) image for anti-CAPNL1 antibody (Calpain 1, Large Subunit) (AA 312-326) (ABIN3044292) Anti-Calpain 1 antibody, Western blotting Lane 1: Cell Lysate Lane 2: COLO320 Cell L...
Immunohistochemistry (IHC) image for anti-CAPNL1 antibody (Calpain 1, Large Subunit) (AA 312-326) (ABIN3044292) Anti-Calpain 1 antibody, IHC(P) IHC(P): Human Intestinal Cancer Tissue