CAPNL1 antibody

Catalog No. ABIN6940954

The Mouse Monoclonal anti-CAPNL1 antibody is suitable to detect CAPNL1 in samples from Human. It has been validated for IHC and ELISA.

Quick Overview for CAPNL1 antibody (ABIN6940954)

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAPNL1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: CAPN1-1530

Product Details anti-CAPNL1 Antibody

Purpose: Calpain 1 Antibody

Specificity: Calpain 1 is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpain 1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human CAPN1 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Skin or Tonsil.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CAPNL1

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Alternative Name: CAPN1

Background:

Calcium dependent protease small subunit 1, Calcium-activated neutral proteinase 1, Calpain 1 large subunit, Calpain mu-type, Calpain regulatory subunit, Calpain small subunit 1, Calpain, large polypeptide L1, Calpain-1 catalytic subunit, CANP small subunit, CANP1, CANPL1, CAPN1, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, Mu Calpain

HeLa cells. Skin or Tonsil.

Molecular Weight: ~80kDa

Gene ID: 823, 502842

UniProt: P07384

Pathways: Apoptosis