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anti-Mouse (Murine) Caspase 10 antibody for Immunohistochemistry

Recommended Caspase 10 Antibody (supplied by: Log in to see )

Antigen
Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10) Antibodies
  • alps2
  • ALPS2
  • caspace-10
  • caspase-10
  • FLICE2
  • flice2
  • MCH4
  • mch4
  • xCaspace-10
  • xCaspase-10
Epitope
N-Term
28
8
5
5
5
4
4
4
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
139
20
18
1
Host
Rabbit
124
9
3
2
1
Clonality
Monoclonal
Conjugate
This Caspase 10 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
110
45
32
21
19
11
8
7
3
2
2
1
1
Supplier
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Available images

Catalog No. ABIN189198
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22865479 ABIN2704575 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.22865479 ABIN2430935 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22865479 ABIN2427581 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0011340706 ABIN1584152 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011340706 ABIN3020701 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011340706 ABIN1584151 IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011340706 ABIN407612 ICC IF IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal

Similar anti-Caspase 10 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 8 Antibodies 45 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 10 Antibodies
Immunofluorescence (IF) 5 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 8 Antibodies 34 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Western Blotting (WB) 18 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies

General

Antigen Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10) Antibodies
Epitope N-Term
(28), (8), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(139), (20), (18), (1)
Host Rabbit
(124), (9), (3), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 10 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(110), (45), (32), (21), (19), (11), (8), (7), (3), (2), (2), (1), (1)
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Product Details anti-Caspase 10 Antibody

Target Details Caspase 10 Application Details Handling Images
Specificity The antibody does not cross-react with other Caspase family members.
No Cross-Reactivity Rat (Rattus)
Purification IgG purified
Immunogen A synthetic peptide corresponding to N-terminal residues of human Caspase-10 subunit p23/17 was used as immunogen.
Clone E35
Isotype IgG

Target Details Caspase 10

Product Details anti-Caspase 10 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Caspase-10 (CASP10 Antibody Abstract)
Background Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in theinitiation and execution of apoptosis. Caspase-10 (MCH4, FLICE2) is one of the initiatorcaspases (1,2), and is localized to various tissues. Pro-caspase-10 has two deatheffector domains (DEDs) that bind FADD and recruits both TNFR1 and CD95 to formcomplexes with these receptors and induce apoptosis (1,2). This death domain complexcleaves pro-caspase-10 into a large active fragment and a small fragment. Cleavedcaspase-10 leads to the processing of caspase-3 and caspase-7, initiating a caspasecascade and subsequent apoptosis (3,4). It has been shown that the Caspase-10protein is not produced in neither mouse nor rat (5). Alternate Names: anti-Apoptosis related cysteine peptidase antibody, anti-Apoptotic protease Mch 4antibody, anti-Apoptotic protease Mch4 antibody, anti-CASP 10 antibody, anti-CASP10antibody, anti-Caspase 10 antibody, anti-FAS associated death domain proteininterleukin 1B converting enzyme 2 antibody, anti-FLICE2 antibody, anti-ICE likeapoptotic protease 4 antibody.
Gene Symbol: CASP10
Gene ID 843
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Caspase 10 Antibody Target Details Caspase 10 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 59kDa.
Recommended dilutions: Flow Cytometry 1:50, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:50-1:100, Immunoprecipitation 1:10, Western Blot 1:1000
Protocol Protocol specific for pro Caspase 10 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-Caspase 10 Antibody Target Details Caspase 10 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-Caspase 10 Antibody Target Details Caspase 10 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 10 antibody (Caspase 10, Apoptosis-Related Cysteine Peptidase) (N-Term) (ABIN189198) Western blot analysis of HeLa cell lysate using anti-Pro-Caspase-10 RabMab (ABIN18919...
Immunohistochemistry (IHC) image for anti-Caspase 10 antibody (Caspase 10, Apoptosis-Related Cysteine Peptidase) (N-Term) (ABIN189198) Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-C...
Immunofluorescence (IF) image for anti-Caspase 10 antibody (Caspase 10, Apoptosis-Related Cysteine Peptidase) (N-Term) (ABIN189198) Caspase 10 expression in apoptotic HeLa cells detected by Immunofluorescent staining ...