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|Antigen||Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) Antibodies|
|Epitope||AA 32-169 Alternatives|
|Conjugate||This Caspase 2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-Caspase 2 AntibodyTarget Details Caspase 2 Application Details Handling Images
|Specificity||The antibody recognizes human caspase-2. Other specis not tested.|
|Characteristics||Synonyms: CASP-2, CASP2, ICH1, ICH-1 protease, NEDD2, Neural precursor cell expresseddevelopmentally down-regulated protein 2|
|Purification||Protein-G affinity chromatography|
|Immunogen||Recombinant human caspase-2 (32-169 aa) purified from E. coli. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0myeloma cell line|
Target Details Caspase 2Product Details anti-Caspase 2 Antibody Application Details Handling Images back to top
|Alternative Name||Caspase-2 (CASP2 Antibody Abstract)|
|Background||The caspase family of cystein proteases functions as fundamental effectors in apoptosis. Caspase-2 is required for the permeabilisation of the mitochondria and the resulting amplification of caspase activity, in response to cytotoxic stress. Cytotoxic stress induced proteolytic cleavage of the caspase-2 proenzyme forms an active caspase which cleaves other caspases in a cascade. This leads to the release of cytochrome c and Smac from mitochondria and the translocation of Bax from the cytoplasm to the mitochondria, leading to apoptosis. In adults, caspase-2 is expressed in the placenta, lung, kidney, pancreas and at lower levels in the heart, brain, liver and skeletal muscle.Synonyms: CASP-2, CASP2, ICH-1 protease, ICH1, NEDD2, Neural precursor cell expressed developmentally down-regulated protein 2|
|Research Area||Apoptosis/Necrosis, Cancer, Autophagy|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-Caspase 2 Antibody Target Details Caspase 2 Handling Images back to top
ELISA. Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Caspase 2 Antibody Target Details Caspase 2 Application Details Images back to top
|Buffer||PBS, pH 7.4, containing 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store at +4 °C or at -20 °C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-Caspase 2 Antibody Target Details Caspase 2 Application Details Handling back to top