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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||13 Antibodies||28 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||4 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies||7 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies||10 Antibodies|
|Immunofluorescence (IF)||4 Antibodies||4 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||24 Antibodies||26 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||10 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Western Blotting (WB)||51 Antibodies|
|Antigen||Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Caspase 8 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-Caspase 8 AntibodyTarget Details Caspase 8 Application Details Handling References for anti-Caspase 8 Antibody (ABIN499573) Images
|Specificity||Caspase-8 antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human Caspase-8 isoform A.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||Virus Casp-8 / FLICE / MACH / Mch5 (C-Terminus) Peptide|
Target Details Caspase 8Product Details anti-Caspase 8 Antibody Application Details Handling References for anti-Caspase 8 Antibody (ABIN499573) Images back to top
|Alternative Name||Caspase-8 (CASP8 Antibody Abstract)|
|Background||Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1 and 2). The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death (reviewed in 3). Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex (4). The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain, subsequent oligomerization promotes procaspase-8 autoactivation (reviewed in 5). FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis (6).Synonyms: Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, CASP8, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MCH5, MORT1-associated CED-3 homolog|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling|
Application DetailsProduct Details anti-Caspase 8 Antibody Target Details Caspase 8 Handling References for anti-Caspase 8 Antibody (ABIN499573) Images back to top
ELISA. Western Blot: Caspase-8 antibody can be used for the detection of caspase-8 at 0.5 μg/mL. Depending on cell lines or tissues used, either full-length or other cleavage products maybe observed. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details References for anti-Caspase 8 Antibody (ABIN499573) Images back to top
|Buffer||PBS containing 0.02 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
References for anti-Caspase 8 Antibody (ABIN499573)Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling Images back to top
Zhivotovsky, Orrenius: "Caspase-2 function in response to DNA damage." in: Biochemical and biophysical research communications, Vol. 331, Issue 3, pp. 859-67, 2005 (PubMed).
Martinon, Tschopp: "Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases." in: Cell, Vol. 117, Issue 5, pp. 561-74, 2004 (PubMed).
Wolf, Green: "Suicidal tendencies: apoptotic cell death by caspase family proteinases." in: The Journal of biological chemistry, Vol. 274, Issue 29, pp. 20049-52, 1999 (PubMed).
Muzio, Chinnaiyan, Kischkel et al.: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996 (PubMed).
ImagesProduct Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling References for anti-Caspase 8 Antibody (ABIN499573) back to top