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anti-Rat (Rattus) Caspase 8 antibody for Enzyme Immunoassay

Recommended Caspase 8 Antibody (supplied by: Log in to see )

Antigen
Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
  • ALPS2B
  • CAP4
  • Casp-8
  • CASP-8
  • casp8
  • CASP8
  • casp8-A
  • caspase-8
  • FLICE
  • MACH
  • Mch5
  • MCH5
  • xCaspase-8
  • zgc:92075
Epitope
C-Term
47
18
18
17
13
10
10
8
8
7
7
6
6
5
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
342
95
68
19
18
14
4
1
1
1
Host
Rabbit
271
104
11
2
Clonality
Polyclonal
Conjugate
This Caspase 8 antibody is un-conjugated
18
12
8
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
326
114
81
70
42
34
31
23
12
11
7
5
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN499573
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN1105678 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 1H11

Top referenced anti-Caspase 8 antibody for Enzyme Immunoassay

Similar anti-Caspase 8 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 15 Antibodies 30 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 4 Antibodies
Flow Cytometry (FACS) 4 Antibodies 8 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 11 Antibodies
Immunofluorescence (IF) 5 Antibodies 5 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 29 Antibodies 31 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 55 Antibodies

General

Antigen Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
Epitope C-Term
(47), (18), (18), (17), (13), (10), (10), (8), (8), (7), (7), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(342), (95), (68), (19), (18), (14), (4), (1), (1), (1)
Host Rabbit
(271), (104), (11), (2)
Clonality
Polyclonal
Conjugate This Caspase 8 antibody is un-conjugated
(18), (12), (8), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(326), (114), (81), (70), (42), (34), (31), (23), (12), (11), (7), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Caspase 8 Antibody

Target Details Caspase 8 Application Details Handling References for anti-Caspase 8 Antibody (ABIN499573) Images
Specificity Caspase-8 antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human Caspase-8 isoform A.
Purification Affinity chromatography purified via peptide column
Immunogen Virus Casp-8 / FLICE / MACH / Mch5 (C-Terminus) Peptide
Isotype IgG

Target Details Caspase 8

Product Details anti-Caspase 8 Antibody Application Details Handling References for anti-Caspase 8 Antibody (ABIN499573) Images back to top
Antigen
Alternative Name Caspase-8 (CASP8 Antibody Abstract)
Background Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1 and 2). The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death (reviewed in 3). Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex (4). The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain, subsequent oligomerization promotes procaspase-8 autoactivation (reviewed in 5). FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis (6).Synonyms: Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, CASP8, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MCH5, MORT1-associated CED-3 homolog
Gene ID 841
NCBI Accession NP_001219
UniProt Q14790
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling

Application Details

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Handling References for anti-Caspase 8 Antibody (ABIN499573) Images back to top
Application Notes ELISA. Western Blot: Caspase-8 antibody can be used for the detection of caspase-8 at 0.5 μg/mL. Depending on cell lines or tissues used, either full-length or other cleavage products maybe observed. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details References for anti-Caspase 8 Antibody (ABIN499573) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-Caspase 8 Antibody (ABIN499573)

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling Images back to top
Background publications

Zhivotovsky, Orrenius: "Caspase-2 function in response to DNA damage." in: Biochemical and biophysical research communications, Vol. 331, Issue 3, pp. 859-67, 2005

Martinon, Tschopp: "Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases." in: Cell, Vol. 117, Issue 5, pp. 561-74, 2004

Wolf, Green: "Suicidal tendencies: apoptotic cell death by caspase family proteinases." in: The Journal of biological chemistry, Vol. 274, Issue 29, pp. 20049-52, 1999

Muzio, Chinnaiyan, Kischkel, ORourke, Shevchenko, Ni, Scaffidi, Bretz, Zhang, Gentz, Mann, Krammer, Peter, Dixit: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996

Images

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling References for anti-Caspase 8 Antibody (ABIN499573) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (C-Term) (ABIN499573) Immunocytochemistry of caspase-8 in Jurkat cells with AP30203PU-N caspase-8 antibody ...
Western Blotting (WB) image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (C-Term) (ABIN499573) Western blot analysis of caspase-8 in Jurkat cell lysate with AP30203PU-N caspase-8 a...