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anti-Human Clusterin antibody for Immunocytochemistry

Recommended Clusterin Antibody (supplied by: Log in to see )

Antigen
Clusterin (CLU) Antibodies
  • 40
  • AI893575
  • APO-J
  • APOJ
  • ApoJ
  • Cli
  • CLI
  • CLU
  • clu
  • CLU1
  • CLU2
  • D14Ucla3
  • DAG
  • GP80
  • gpIII
  • KUB1
  • LOC100136120
  • MGC53055
  • MGC89163
  • NA1/NA2
  • RATTRPM2B
  • SGP-2
  • Sgp-2
  • SGP2
  • Sgp2
  • SP-40
  • SP40
  • Sugp-2
  • TRPM-2
  • Trpm2
  • TRPM2
  • TRPM2B
  • Trpmb
  • xclu
  • zgc:73352
Reactivity
Human
294
59
55
19
18
5
4
3
2
1
Host
Mouse
164
139
72
8
5
2
Clonality
Monoclonal
Conjugate
This Clusterin antibody is un-conjugated
32
26
13
11
7
7
3
3
3
3
3
3
3
3
3
3
3
2
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
317
264
135
99
58
53
34
29
28
11
7
1
1
Supplier
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Catalog No. ABIN108488
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010022576 ABIN2469990 ICC IHC ELISA Mouse IgG1 Log in to see Hs-3
0.0010022576 ABIN239817 ICC IHC (p) ELISA Mouse IgG1 Log in to see Hs-3
0.0010022576 ABIN94264 ICC IHC (p) ELISA WB Mouse IgG1 Log in to see Hs-3 6
0.0010022576 ABIN3201572 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 228-449 Log in to see
0.0010022576 ABIN4299260 ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see CLI-9 2
0.0010022576 ABIN1077937 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 228-449 Log in to see Polyclonal
0.0010022576 ABIN1723370 ICC IHC (p) ELISA Mouse IgG1 Log in to see Hs-3
0.0010022576 ABIN323977 ICC IF IHC ELISA WB Mouse IgG1 Log in to see
0.0010022576 ABIN1839608 ICC ELISA WB Mouse IgG1 Log in to see Hs-3
0.0010022576 ABIN594376 ICC ELISA WB Mouse IgG1 Log in to see
0.0010022576 ABIN296850 ICC IP IHC WB Mouse IgG1 Log in to see 2E9C2
0.0010022576 ABIN2595546 ICC IF IHC ELISA WB Mouse IgG1 Log in to see s-3
0.0010022576 ABIN2157411 ICC IP IHC WB Mouse IgG1 Log in to see CLI-9
0.0010022576 ABIN4299258 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0010022576 ABIN4299256 ELISA ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see Hs-3
0.0010022576 ABIN4299261 ELISA ICC IF IHC IHC (p) WB DyLight 350 Mouse IgG1 Log in to see Hs-3
0.0010022576 ABIN4299264 ELISA ICC IF IHC IHC (p) WB PerCP Mouse IgG1 Log in to see Hs-3
0.0010022576 ABIN4299265 ELISA ICC IF IHC IHC (p) WB Allophycocyanin Mouse IgG1 Log in to see Hs-3
0.0010022576 ABIN4299267 ELISA ICC IF IHC IHC (p) WB DyLight 680 Mouse IgG1 Log in to see Hs-3
0.0010022576 ABIN4299269 ELISA ICC IF IHC IHC (p) WB DyLight 405LS Mouse IgG1 Log in to see Hs-3

Top referenced anti-Clusterin antibody for Immunocytochemistry

Similar anti-Clusterin Antibodies

Application / Reactivity Human
ELISA 195 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 28 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 45 Antibodies
Immunofluorescence (IF) 50 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 125 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 24 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 88 Antibodies
Immunoprecipitation (IP) 28 Antibodies
Western Blotting (WB) 253 Antibodies

General

Antigen Clusterin (CLU) Antibodies
Reactivity Human
(294), (59), (55), (19), (18), (5), (4), (3), (2), (1)
Host Mouse
(164), (139), (72), (8), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Clusterin antibody is un-conjugated
(32), (26), (13), (11), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(317), (264), (135), (99), (58), (53), (34), (29), (28), (11), (7), (1), (1)
Pubmed 4 references available
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Product Details anti-Clusterin Antibody

Target Details Clusterin Application Details Handling References for anti-Clusterin Antibody (ABIN108488) Images
No Cross-Reactivity Cat (Feline), Dog (Canine), Cow (Bovine)
Cross-Reactivity (Details) Not yet tested in other species.
Purification Affinity chromatography on a column with immobilized protein A-Sepharose followed by DEAE-chromatography.
Immunogen
  • The antibody is a mouse monoclonal antibody against Human Clusterin.
  • Source of Immunogen: The antibody was prepared against Triton X-100 human sperm extract.
Clone Hs-3
Isotype IgG1

Target Details Clusterin

Product Details anti-Clusterin Antibody Application Details Handling References for anti-Clusterin Antibody (ABIN108488) Images back to top
Antigen
Alternative Name Clusterin (CLU Antibody Abstract)
Background
  • Clusterin is a 75-80 kD disulfide-linked heterodimeric protein containing about 30 % of N-linked carbohydrate rich in sialic acid, but truncated forms targeted to the nucleus have also been identified. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the alpha and beta chains. These are assembled anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil alpha-helices and three predicted amphipathic alpha-helices. The six sites of N-linked glycosylation are indicated as yellow spots. Across a broad range of species clusterin shows 70 % to 80 % of sequence homology. It is ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodelling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition. A detailed mechanism of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced by degraded and misfolded protein precipitates.Clus-terin is up- or downregulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.
Research Area Receptors, Cancer
Pathways Apoptosis

Application Details

Product Details anti-Clusterin Antibody Target Details Clusterin Handling References for anti-Clusterin Antibody (ABIN108488) Images back to top
Application Notes
  • Quality control: SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Clusterin Antibody Target Details Clusterin Application Details References for anti-Clusterin Antibody (ABIN108488) Images back to top
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2, 15 mM SODIUM AZIDE.
Preservative Sodium azide
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label

References for anti-Clusterin Antibody (ABIN108488)

Product Details anti-Clusterin Antibody Target Details Clusterin Application Details Handling Images back to top
Background publications

Zenkel, Kruse, Jünemann, Naumann, Schlötzer-Schrehardt: "Clusterin deficiency in eyes with pseudoexfoliation syndrome may be implicated in the aggregation and deposition of pseudoexfoliative material." in: Investigative ophthalmology & visual science, Vol. 47, Issue 5, pp. 1982-90, 2006

Zhou W,Janulis L,Park II,Lee C: "A novel anti-proliferative property of clusterin in prostate cancer cells." in: Life Sci, Vol. 72, Issue 1, pp. 11-21, 2002

Jones, Jomary: "Clusterin." in: The international journal of biochemistry & cell biology, Vol. 34, Issue 5, pp. 427-31, 2002

Connor JR,Kumar S,Sathe G,Mooney J,O'Brien SP,Mui P,Murdock PR,Gowen M,Lark MW: "Clusterin expression in adult human normal and osteoarthritic articular cartilage." in: Osteoarthritis Cartilage, Vol. 9, Issue 8, pp. 727-37, 2001