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|Antigen||Clusterin (CLU) Antibodies|
|Conjugate||This Clusterin antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
|3 references available|
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Product Details anti-Clusterin AntibodyTarget Details Clusterin Application Details Handling References for anti-Clusterin antibody (ABIN114700) Images
|Specificity||The anti-Clusterin Antibody, Clone Hs-3 is a Mouse monoclonal antibody against Human Clusterin. Does not react with Cat, Dog, Bovine.|
|Purification||Affinity chromatography on protein A followed by DEAE-chromatography|
|Immunogen||The antibody was prepared against Triton X-100 human sperm extract.|
Target Details ClusterinProduct Details anti-Clusterin Antibody Application Details Handling References for anti-Clusterin antibody (ABIN114700) Images back to top
|Alternative Name||Apolipoprotein J (Apo J) (CLU Antibody Abstract)|
|Background||Clusterin is a 75-80 kD disulfide-linked heterodimeric protein containing about 30 % of N-linked carbohydrate rich in sialic acid, but truncated forms targeted to the nucleus have also been identified. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the alpha and beta chains. These are assembled antiparallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil alpha-helices and three predicted amphipathic alpha-helices. The six sites of N-linked glycosylation are indicated as yellow spots. Across a broad range of species clusterin shows 70 % to 80 % of sequence homology. It is ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodelling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition. A detailed mechanism of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced by degraded and misfolded protein precipitates.Clusterin is up- or downregulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2|
|Research Area||Metabolism, Receptors|
|Pathways||Apoptosis, Negative Regulation of intrinsic apoptotic Signaling|
Application DetailsProduct Details anti-Clusterin Antibody Target Details Clusterin Handling References for anti-Clusterin antibody (ABIN114700) Images back to top
ELISA. Immunocytochemistry. Immunofluorescence. Immunohistochemistry. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Clusterin Antibody Target Details Clusterin Application Details References for anti-Clusterin antibody (ABIN114700) Images back to top
|Reconstitution||Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.|
|Buffer||0.05 M Phosphate buffer, 0.5 M NaCl, pH 7.2, 15 mM Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.|
|Expiry Date||6 months|
References for anti-Clusterin antibody (ABIN114700)Product Details anti-Clusterin Antibody Target Details Clusterin Application Details Handling Images back to top
|Product cited in:||
Schlötzer-Schrehardt, Pasutto, Sommer, Hornstra, Kruse, Naumann, Reis, Zenkel: "Genotype-correlated expression of lysyl oxidase-like 1 in ocular tissues of patients with pseudoexfoliation syndrome/glaucoma and normal patients." in: The American journal of pathology, Vol. 173, Issue 6, pp. 1724-35, 2008
Zhang, Sha, Wood, Galindo, Garner, Burkart, Suarez, Sierra, Agar, Peterson, Chopra: "Alteration in the activation state of new inflammation-associated targets by phospholipase A2-activating protein (PLAA)." in: Cellular signalling, Vol. 20, Issue 5, pp. 844-61, 2008
Zenkel, Kruse, Jünemann, Naumann, Schlötzer-Schrehardt: "Clusterin deficiency in eyes with pseudoexfoliation syndrome may be implicated in the aggregation and deposition of pseudoexfoliative material." in: Investigative ophthalmology & visual science, Vol. 47, Issue 5, pp. 1982-90, 2006