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anti-Human Clusterin antibody for Immunohistochemistry (Frozen Sections)

Recommended Clusterin Antibody (supplied by: Log in to see )

Antigen
Clusterin (CLU) Antibodies
  • LOC100136120
  • clu
  • MGC53055
  • zgc:73352
  • xclu
  • MGC89163
  • CLU
  • APO-J
  • APOJ
  • CLI
  • CLU1
  • CLU2
  • KUB1
  • NA1/NA2
  • SGP-2
  • SGP2
  • SP-40
  • TRPM-2
  • TRPM2
  • DAG
  • RATTRPM2B
  • SP40
  • TRPM2B
  • Trpm2
  • Trpmb
  • AI893575
  • ApoJ
  • Cli
  • D14Ucla3
  • Sgp-2
  • Sgp2
  • Sugp-2
  • GP80
  • gpIII
  • 40
  • clusterin-1
  • clusterin
  • Clusterin
  • LOC100136120
  • clu
  • CLU
  • clus
  • Clu
Reactivity
Human
288
62
59
18
17
5
3
3
3
2
1
1
1
Host
Mouse
163
135
73
8
5
2
Clonality
Monoclonal
Conjugate
This Clusterin antibody is un-conjugated
32
26
12
11
7
7
3
3
3
3
3
3
3
3
3
3
3
2
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
344
269
155
104
56
51
28
27
26
15
10
7
1
Supplier
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Catalog No. ABIN114700
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN181112 EIA FACS IHC (fro) WB Mouse IgG1 Log in to see 3R3-2 2
1 ABIN4299260 ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see CLI-9 2
1 ABIN967369 IHC (fro) IHC (p) WB Mouse IgG1, kappa Log in to see E5 3
1 ABIN187541 IHC (fro) IF IP WB Mouse IgG1 Log in to see CLI-9
1 ABIN3201572 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 228-449 Log in to see
1 ABIN206663 FACS IHC (fro) ELISA WB Mouse IgG1 Log in to see 3R3-2
1 ABIN116548 EIA IHC (fro) IHC (p) IP WB Goat Log in to see Polyclonal
1 ABIN601047 IHC (fro) FACS IHC (p) ELISA WB Mouse IgG1 Log in to see
1 ABIN2473011 IHC (fro) FACS ELISA WB Mouse IgG1 Log in to see 3R3-2 2
1 ABIN4264121 ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgG1 Log in to see CLI-9
1 ABIN4264122 ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG1 Log in to see CLI-9
1 ABIN4264127 ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 Log in to see CLI-9
1 ABIN4264124 ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 Log in to see CLI-9
1 ABIN4264126 ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG1 Log in to see CLI-9
1 ABIN4264129 ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG1 Log in to see CLI-9
1 ABIN4264133 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG1 Log in to see CLI-9
1 ABIN4264134 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 Log in to see CLI-9
1 ABIN4264131 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 Log in to see CLI-9
1 ABIN4264132 ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 Log in to see CLI-9
1 ABIN4264123 ICC IF IHC IHC (fro) IHC (p) IP WB PE Mouse IgG1 Log in to see CLI-9

Top referenced anti-Clusterin antibody for Immunohistochemistry (Frozen Sections)

Similar anti-Clusterin Antibodies

Application / Reactivity Human
ELISA 190 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 28 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 42 Antibodies
Immunofluorescence (IF) 48 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 128 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 23 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 97 Antibodies
Immunoprecipitation (IP) 19 Antibodies
Western Blotting (WB) 253 Antibodies

General

Antigen Clusterin (CLU) Antibodies
Reactivity Human
(288), (62), (59), (18), (17), (5), (3), (3), (3), (2), (1), (1), (1)
Host Mouse
(163), (135), (73), (8), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Clusterin antibody is un-conjugated
(32), (26), (12), (11), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(344), (269), (155), (104), (56), (51), (28), (27), (26), (15), (10), (7), (1)
Pubmed 3 references available
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Product Details anti-Clusterin Antibody

Target Details Clusterin Application Details Handling References for anti-Clusterin Antibody (ABIN114700) Images
Specificity The anti-Clusterin Antibody, Clone Hs-3 is a Mouse monoclonal antibody against Human Clusterin. Does not react with Cat, Dog, Bovine.
Purification Affinity chromatography on protein A followed by DEAE-chromatography
Immunogen The antibody was prepared against Triton X-100 human sperm extract.
Clone Hs-3
Isotype IgG1

Target Details Clusterin

Product Details anti-Clusterin Antibody Application Details Handling References for anti-Clusterin Antibody (ABIN114700) Images back to top
Antigen
Alternative Name Apolipoprotein J (Apo J) (CLU Antibody Abstract)
Background Clusterin is a 75-80 kD disulfide-linked heterodimeric protein containing about 30 % of N-linked carbohydrate rich in sialic acid, but truncated forms targeted to the nucleus have also been identified. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the alpha and beta chains. These are assembled antiparallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil alpha-helices and three predicted amphipathic alpha-helices. The six sites of N-linked glycosylation are indicated as yellow spots. Across a broad range of species clusterin shows 70 % to 80 % of sequence homology. It is ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodelling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition. A detailed mechanism of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced by degraded and misfolded protein precipitates.Clusterin is up- or downregulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2
Gene ID 1191
NCBI Accession NP_976084
UniProt P10909
Research Area Metabolism, Receptors
Pathways Apoptosis, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-Clusterin Antibody Target Details Clusterin Handling References for anti-Clusterin Antibody (ABIN114700) Images back to top
Application Notes ELISA. Immunocytochemistry. Immunofluorescence. Immunohistochemistry. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Clusterin Antibody Target Details Clusterin Application Details References for anti-Clusterin Antibody (ABIN114700) Images back to top
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Concentration 1.0 mg/mL
Buffer 0.05 M Phosphate buffer, 0.5 M NaCl, pH 7.2, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
Expiry Date 6 months

References for anti-Clusterin Antibody (ABIN114700)

Product Details anti-Clusterin Antibody Target Details Clusterin Application Details Handling Images back to top
Product cited in:

Schlötzer-Schrehardt, Pasutto, Sommer, Hornstra, Kruse, Naumann, Reis, Zenkel: "Genotype-correlated expression of lysyl oxidase-like 1 in ocular tissues of patients with pseudoexfoliation syndrome/glaucoma and normal patients." in: The American journal of pathology, Vol. 173, Issue 6, pp. 1724-35, 2008

Zhang, Sha, Wood, Galindo, Garner, Burkart, Suarez, Sierra, Agar, Peterson, Chopra: "Alteration in the activation state of new inflammation-associated targets by phospholipase A2-activating protein (PLAA)." in: Cellular signalling, Vol. 20, Issue 5, pp. 844-61, 2008

Background publications

Zenkel, Kruse, Jünemann, Naumann, Schlötzer-Schrehardt: "Clusterin deficiency in eyes with pseudoexfoliation syndrome may be implicated in the aggregation and deposition of pseudoexfoliative material." in: Investigative ophthalmology & visual science, Vol. 47, Issue 5, pp. 1982-90, 2006